We made a series of improved Gateway binary vectors (pGWBs) for plant transformation.
Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6ÂHis …

We developed a new series of binary vectors useful for Gateway cloning to facilitate
transgenic experiments in plant biotechnology. The new system, Gateway Binary Vectors …

We developed a new series of Gateway binary vectors, R4pGWBs, that are plant
transformation vectors designed for one-step construction of chimeric genes between any …

The availability of complete plant genome sequences has opened a new era of plant
molecular research using approaches such as comprehensive expression analysis, ectopic …

Background In plant functional genomic studies, gene cloning into binary vectors for plant
transformation is a routine procedure. Traditionally, gene cloning has relied on restriction …

The study of biological systems relies to a large extent on DNA cloning technologies
enabling the analysis of recombinant genes through transgenic research. In this context, the …

Background The progress and completion of various plant genome sequencing projects has
paved the way for diverse functional genomic studies that involve cloning, modification and …

Gateway cloning technology facilitates high‐throughput cloning of target sequences by
making use of the bacteriophage lambda site‐specific recombination system. Target …

We have constructed a binary vector for Agrobacterium-mediated plant transformation,
which has a multiple cloning site consisting of 13 hexanucleotide restriction sites, 6 …

Agrobacterium tumefaciens is the preferred method for transformation of a wide range of
plant species. Commonly, the genes to be transferred are cloned between the left and right T …