Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus

VG Panferov, IV Safenkova, NA Byzova… - Food and Agricultural …, 2018 - Taylor & Francis
Rapid non-laboratory screening of plants for pathogenic viruses crucially influences crop
yields in modern agricultural technologies. The aim of this study was to develop a highly …

How to improve sensitivity of sandwich lateral flow immunoassay for corpuscular antigens on the example of potato virus Y?

SC Razo, VG Panferov, IV Safenkova, YA Varitsev… - Sensors, 2018 - mdpi.com
A simple approach was proposed to decrease the detection limit of sandwich lateral flow
immunoassay (LFIA) by changing the conditions for binding between a polyvalent antigen …

Double-enhanced lateral flow immunoassay for potato virus X based on a combination of magnetic and gold nanoparticles

SC Razo, VG Panferov, IV Safenkova, YA Varitsev… - Analytica chimica …, 2018 - Elsevier
This study presents the joint use of magnetic nanoparticles (MNPs) and gold nanoparticles
(GNPs) for double enhancement in a lateral flow immunoassay (LFIA). The study realizes …

Alarm lateral flow immunoassay for detection of the total infection caused by the five viruses

IV Safenkova, VG Panferov, NA Panferova, YA Varitsev… - Talanta, 2019 - Elsevier
This study presents new type of the lateral flow immunoassay (LFIA) for multi-target analysis.
A test, named alarm-LFIA, has an essentially new function that consists in notice (signaling …

Post-assay growth of gold nanoparticles as a tool for highly sensitive lateral flow immunoassay. Application to the detection of potato virus X

VG Panferov, IV Safenkova, AV Zherdev, BB Dzantiev - Microchimica Acta, 2018 - Springer
This article demonstrates a new kind of a highly sensitive lateral flow immunoassay (LFIA). It
is based on the enlargement of the size of gold nanoparticles (GNPs) directly on the test strip …

Assessment of Performance Ability of Three Diagnostic Methods for Detection of Potato Leafroll Virus (PLRV) Using Different Visualizing Systems

MA Almasi, A Moradi, J Nasiri, S Karami… - Applied biochemistry and …, 2012 - Springer
To diminish the time required for some diagnostic assays including reverse transcription
PCR (RT-PCR), reverse transcription loop-mediated isothermal amplification (RT-LAMP; …

Multiplex microsphere immuno-detection of potato virus Y, X and PLRV

JHW Bergervoet, J Peters… - Journal of Virological …, 2008 - Elsevier
To monitor seed potatoes for potato virus X, Y and PLRV, a multiplex microsphere
immunoassay (MIA) was developed based on the Luminex xMAP technology, as an …

Enlargement of gold nanoparticles for sensitive immunochromatographic diagnostics of potato brown rot

SC Razo, NA Panferova, VG Panferov, IV Safenkova… - Sensors, 2019 - mdpi.com
Lateral flow immunoassay (LFIA) is a convenient tool for rapid field-based control of various
bacterial targets. However, for many applications, the detection limits obtained by LFIA are …

[PDF][PDF] Detection of coat protein gene of the Potato leafroll virus by reverse transcription loop-mediated isothermal amplification

MA Almasi, H Jafary, A Moradi, N Zand… - J Plant Pathol …, 2013 - academia.edu
Loop-mediated isothermal amplification assay amplifies DNA/RNA with high specificity and
sensitivity. In this study, we describe an optimized reverse transcription-LAMP assay for …

Setting up the cut-off level of a sensitive barcode lateral flow assay with magnetic nanoparticles

VG Panferov, IV Safenkova, AV Zherdev, BB Dzantiev - Talanta, 2017 - Elsevier
A barcode lateral flow immunoassay (LFIA) based on magnetic nanoparticles with a
controllable cut-off level was developed for the first time. The regulation of the cut-off levels …