The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO2

T Gassler, M Sauer, B Gasser, M Egermeier… - Nature …, 2020 - nature.com
The methylotrophic yeast Pichia pastoris is widely used in the manufacture of industrial
enzymes and pharmaceuticals. Like most biotechnological production hosts, P. pastoris is …

[HTML][HTML] Genome sequence of the recombinant protein production host Pichia pastoris

K De Schutter, YC Lin, P Tiels, A Van Hecke… - Nature …, 2009 - nature.com
The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as
a model organism for studying peroxisomal biogenesis and methanol assimilation. P …

Conversion of CO2 into organic acids by engineered autotrophic yeast

M Baumschabl, Ö Ata, BM Mitic, L Lutz… - Proceedings of the …, 2022 - National Acad Sciences
The increase of CO2 emissions due to human activity is one of the preeminent reasons for
the present climate crisis. In addition, considering the increasing demand for renewable …

Towards systems metabolic engineering in Pichia pastoris

JP Schwarzhans, T Luttermann, M Geier… - Biotechnology …, 2017 - Elsevier
The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of
recombinant proteins, frequently outperforming other heterologous hosts. Already, a …

The yeast stands alone: the future of protein biologic production

KR Love, NC Dalvie, JC Love - Current opinion in biotechnology, 2018 - Elsevier
Highlights•Yeasts create agility in manufacturing due to high titers and small time
scales.•Knowledge of host biology enables 'fit-to-purpose'strains for specific products.•Pichia …

[图书][B] Pichia protocols

DR Higgins, JM Cregg - 1998 - Springer
The past five years have seen an exponenttal growth m the use of the methylotrophic yeast
Pichza pastorzs as a heterologous protein expression system. The exrstence of yeasts that …

[HTML][HTML] Metabolic engineering of Pichia pastoris

DA Peña, B Gasser, J Zanghellini, MG Steiger… - Metabolic …, 2018 - Elsevier
Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

High cell-density fermentation

J Stratton, V Chiruvolu, M Meagher - Pichia protocols, 1998 - Springer
The purpose of this chapter is to provide sufficient instruction for the reader to implement
fermentation strategies to produce recombinant proteins in the yeast Pichia pastoris. P …

Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris

P Cai, X Wu, J Deng, L Gao, Y Shen… - Proceedings of the …, 2022 - National Acad Sciences
Methanol-based biorefinery is a promising strategy to achieve carbon neutrality goals by
linking CO2 capture and solar energy storage. As a typical methylotroph, Pichia pastoris …

Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris

M Weidner, M Taupp, SJ Hallam - JoVE (Journal of Visualized …, 2010 - jove.com
Protein expression in the microbial eukaryotic host Pichia pastoris offers the possibility to
generate high amounts of recombinant protein in a fast and easy to use expression system …