Challenges of up-scaling lentivirus production and processing

A McCarron, M Donnelley, C McIntyre… - Journal of biotechnology, 2016 - Elsevier
Lentiviruses are becoming an increasingly popular choice of gene transfer vehicle for use in
the treatment of a variety of genetic and acquired human diseases. As research progresses …

Large-scale production of lentiviral vectors: current perspectives and challenges

E Martínez-Molina, C Chocarro-Wrona… - Pharmaceutics, 2020 - mdpi.com
Lentiviral vectors (LVs) have gained value over recent years as gene carriers in gene
therapy. These viral vectors are safer than what was previously being used for gene transfer …

Development of large-scale downstream processing for lentiviral vectors

AJ Valkama, I Oruetxebarria, EM Lipponen… - … Therapy-Methods & …, 2020 - cell.com
The interest in lentiviral vectors (LVs) has increased prominently for gene therapy
applications, but few have reached the later stages of clinical trials. The main challenge has …

Advances in lentivirus purification

AS Moreira, DG Cavaco, TQ Faria… - Biotechnology …, 2021 - Wiley Online Library
Lentiviral vectors (LVs) have been increasingly used as a tool for gene and cell therapies
since they can stably integrate the genome in dividing and nondividing cells. LV production …

Manufacture of third-generation lentivirus for preclinical use, with process development considerations for translation to good manufacturing practice

C Gándara, V Affleck, EA Stoll - Human Gene Therapy Methods, 2018 - liebertpub.com
Lentiviral vectors are used in laboratories around the world for in vivo and ex vivo delivery of
gene therapies, and increasingly clinical investigation as well as preclinical applications …

Recent progress in lentiviral vector mass production

S Ansorge, O Henry, A Kamen - Biochemical engineering journal, 2010 - Elsevier
Lentiviral vectors (LV) are promising tools for gene and cell therapy. They are presently used
in several clinical trials as in vivo or ex vivo gene delivery vectors. However their mass …

Production of lentiviral vectors

OW Merten, M Hebben, C Bovolenta - Molecular Therapy-Methods & …, 2016 - cell.com
Lentiviral vectors (LV) have seen considerably increase in use as gene therapy vectors for
the treatment of acquired and inherited diseases. This review presents the state of the art of …

Optimized production and concentration of lentiviral vectors containing large inserts

N Yacoub, M Romanowska… - The Journal of Gene …, 2007 - Wiley Online Library
Generation of high titer lentiviral stocks and efficient virus concentration are central to
maximize the utility of lentiviral technology. Here we evaluate published protocols for …

[HTML][HTML] Large-scale production of lentiviral vectors using multilayer cell factories

N Rout-Pitt, A McCarron, C McIntyre… - Journal of Biological …, 2018 - ncbi.nlm.nih.gov
Lentiviral-mediated gene therapy has been proposed for the treatment of a range of
diseases, and due to its genome integration properties, it offers the potential for long-lasting …

Production of lentiviral vectors by large‐scale transient transfection of suspension cultures and affinity chromatography purification

MM Segura, A Garnier, Y Durocher… - Biotechnology and …, 2007 - Wiley Online Library
The use of lentiviral vectors as gene delivery vehicles has become increasingly popular in
recent years. The growing interest in these vectors has created a strong demand for large …