Cellular compartments that cannot be biochemically isolated are challenging to
characterize. Here we demonstrate the proteomic characterization of the synaptic clefts that …

Neurons build synaptic contacts using different protein combinations that define the
specificity, function, and plasticity potential of synapses; however, the diversity of synaptic …

Highlights•The protein machinery at inhibitory synapses is more complex than
anticipated.•Many new synapse organizers have recently been identified.•Major progress …

Highlights•Proximity labeling enables proteomic interrogation of the molecular components
of subcellular regions as well as protein interaction networks.•Proximity labeling has been …

At each of the brain's vast number of synapses, the presynaptic nerve terminal, synaptic cleft,
and postsynaptic specialization form a transcellular unit to enable efficient transmission of …

The recent success of large-scale industrialized genomic sequencing opens new doors in
studies of biological systems. In the current post-genomic era we must ask how to translate …

Cells are organized into distinct compartments to perform specific tasks with spatial
precision. In neurons, presynaptic specializations are biochemically complex subcellular …

The chemical synapse is a complex machine separated into three parts: presynaptic,
postsynaptic, and cleft. Super-resolution light microscopy has revealed alignment of …

Understanding the origins and evolution of synapses may provide insight into species
diversity and the organization of the brain. Using comparative proteomics and genomics, we …

Our knowledge of the complex synaptic proteome and its relationship to physiological or
pathological conditions is rapidly expanding. This has been greatly accelerated by the …