A better understanding of proteostasis in health and disease requires robust methods to
determine protein half-lives. Here we improve the precision and accuracy of peptide ion …

Protein turnover is critical to cellular physiology as well as to the growth and maintenance of
tissues. The unique synthesis and degradation rates of each protein help to define tissue …

The coordination of protein synthesis and degradation regulating protein abundance is a
fundamental process in cellular homeostasis. Today, mass spectrometry-based …

The turnover of each protein in the mammalian proteome is a functionally important
characteristic. Here, we employed high-resolution mass spectrometry to quantify protein …

The proteome of any system is a dynamic entity, such that the intracellular concentration of a
protein is dictated by the relative rates of synthesis and degradation. In this work, we have …

Measuring the properties of endogenous cell proteins, such as expression level, subcellular
localization, and turnover rates, on a whole proteome level remains a major challenge in the …

In all cells, proteins are continuously synthesized and degraded to maintain protein
homeostasis and modify gene expression levels in response to stimuli. Collectively, the …

Advances in systems biology have allowed for global analyses of mRNA and protein
expression, but large-scale studies of protein dynamics and turnover have not been …

Although bulk protein turnover has been measured with the use of stable isotope labeled
tracers for over half a century, it is only recently that the same approach has become …

Protein dynamics are essential in regulating nearly every cellular event, and aberrant
proteostasis is the source of many diseases. It is extraordinarily difficult to globally study …