CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Assigning functions to genes and learning how to control their expression are part of the
foundation of cell biology and therapeutic development. An efficient and unbiased method to …

A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …

CRISPR-based genetic screens are accelerating biological discovery, but current methods
have inherent limitations. Widely used pooled screens are restricted to simple readouts …

High-throughput genetic screens have become essential tools for studying a wide variety of
biological processes. Here we experimentally compare systems based on clustered …

Next-generation DNA sequencing technologies have led to a massive accumulation of
genomic and transcriptomic data from patients and healthy individuals. The major challenge …

Exciting new technologies are often self-limiting in their rollout, as access to state-of-the-art
instrumentation or the need for years of hands-on experience, for better or worse, ensures …

Genome editing technologies such as clustered regularly interspaced short palindromic
repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

Single-cell CRISPR screens enable the exploration of mammalian gene function and
genetic regulatory networks. However, use of this technology has been limited by reliance …