Achieving sufficient coverage of regulatory phosphorylation sites by mass spectrometry (MS)-
based phosphoproteomics for signaling pathway reconstitution is challenging, especially …

Quantitative phosphoproteomics has transformed investigations of cell signaling, but it
remains challenging to scale the technology for high-throughput analyses. Here we report a …

Phosphoproteomics can provide insights into cellular signaling dynamics. To achieve deep
and robust quantitative phosphoproteomics profiling for minute amounts of sample, we here …

Systematic approaches to studying cellular signaling require phosphoproteomic techniques
that reproducibly measure the same phosphopeptides across multiple replicates, conditions …

Profiling post-translational modifications represents an alternative dimension to gene
expression data in characterizing cellular processes. Many cellular responses to drugs are …

Improving coverage, robustness, and sensitivity is crucial for routine phosphoproteomics
analysis by single-shot liquid chromatography-tandem mass spectrometry (LC-MS/MS) from …

Effective phosphoproteome of nanoscale sample analysis remains a daunting task, primarily
due to significant sample loss associated with non-specific surface adsorption during …

Quantitative phosphoproteomics workflows traditionally involve additional sample labeling
and fractionation steps for accurate and in-depth analysis. Here we report a high-throughput …

Mass spectrometry has transformed the field of cell signaling by enabling global studies of
dynamic protein phosphorylation ('phosphoproteomics'). Recent developments are enabling …

Single-cell resolution analysis of complex biological tissues is fundamental to capture cell-
state heterogeneity and distinct cellular signaling patterns that remain obscured with …