Normal root of Hyoscyamus niger was cultivated in various nutrient conditions to optimize
root growth and scopolamine production. Nitrate or phosphate concentration had little effect …

Adventitious roots were induced from 2-year-old, hyoscyamine rich suspension cultures of
Hyoscyamus niger L. The cultured roots grew well in Linsmaier-Skoog medium containing …

Normal root of Hyoscyamus niger was cultivated in four different culture systems; a flask, a
stirred tank reactor, a mist bioreactor, and a unified mist bioreactor. The scopolamine …

Hyoscyamus niger is an important medicinal plant and reported to be used for the treatment
of various ailments. The present study tested the effect of different elicitor such as chitosan …

The effect of calcium on the morphology, growth, tropane alkaloid production (hyoscyamine
and scopolamine) and plant nutritional element (calcium, magnesium and potassium) …

Scopolamine is a pharmaceutically important tropane alkaloid extensively used as an
anticholinergic agent. Here, we report the simultaneous introduction and overexpression of …

This study aimed to determine the effects of 24-epibrasinolide (EBL) and methyl jasmonate
(MJ) treatments on growth parameters and secondary metabolite synthesis in adventitious …

Untransformed root culture of Hyoscyamus niger could be induced from petiole explants on
solid MS medium supplemented with 2.0 mg l–1 IBA after four weeks of incubation under …

Callus cultures of Hyoscyamus niger L. were initiated from leaf segments cultured on
Murashige and Skoog (MS) medium supplemented with 0.5 mg/L Benzyl Adenine (BA) and …

A circulation culture system was established for the high density root culture of Duboisia
roots. It consists of a vessel for root culture, an aeration tube, a medium reservoir, and a …