Induction of pluripotent stem cells from human fibroblasts has been achieved by the ectopic
expression of two different sets of four genes. However, the mechanism of the pluripotent …

This unit describes how to generate human induced pluripotent stem (iPS) cells and
evaluate the qualities of the generated iPS cells. The methods for establishment and …

Successful reprogramming of differentiated human somatic cells into a pluripotent state
would allow creation of patient-and disease-specific stem cells. We previously reported …

Pluripotent stem cells, such as embryonic stem cells (ESCs), proliferate rapidly while
maintaining pluripotency, namely, the ability to differentiate into various types of cells …

Induced pluripotent stem (iPS) cells can be generated from somatic cells by the forced
expression of four factors, Oct3/4, Sox2, Klf4, and c-Myc. While a great variety of colonies …

Background Somatic cells can be reprogrammed into induced pluripotent stem (iPS) cells.
Recently, selection of fully reprogrammed cells was achieved based on colony morphology …

Reprogramming of mouse and human somatic cells into induced pluripotent stem (iPS) cells
has been made possible with the expression of the transcription factor quartet Oct4, Sox2, c …

The generation of patient-specific pluripotent stem cells has the potential to accelerate the
implementation of stem cells for clinical treatment of degenerative diseases. Technologies …

Following the success in establishing human induced pluripotent stem (iPS) cells, research
into various applications of the cells derived from human iPS cells has begun in earnest. The …

Induced pluripotent stem (iPS) cells can be generated from somatic cells by transduction
with several transcription factors in mouse and human. However, direct reprogramming in …