We isolated a Vibrio vulnificus mutant that was deficient in both metalloprotease and
cytolysin by allelic exchange. The virulence of this mutant in mice and its cytotoxicity for HEp …

Previous work suggested that a metalloprotease, Vvp, may be a virulence factor of Vibrio
vulnificus, which causes severe wound infection and septicemia in humans. To determine …

Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when
bacterial growth reached the late log phase. A similar pattern of expression has been found …

The ca. 51-kDa extracellular cytolysin of Vibrio vulnificus has been proposed as a virulence
factor. We inactivated the structural gene for cytolysin in fully virulent, clinical V. vulnificus …

Thirty-three strains of Vibrio vulnificus of clinical and environmental origin were examined for
production of 12 extracellular enzymes of potential importance to the virulence of this …

MATERIALS AND METHODS Materials. The protease was purified from the culture
supernatant of V. vulnificus L-180 strain as described previously (12), except that the culture …

Vibrio vulnificus is a causative agent of septicemia or wound infection in human and eel;
however, the genetic variation between human and eel isolates has been reported. In the …

Genes encoding the cytotoxin-hemolysin of Vibrio vulnificus were cloned in Escherichia coli
by using the lytic cloning vector, lambda 1059. Subcloning in plasmid pBR325 resulted in …

The gene (vvp) encoding a thermolabile protease of Vibrio vulnificus was cloned and
sequenced. The transcription start point was also determined by primer extension. The …

Vibrio vulnificus is an opportunistic gram-negative pathogen that commonly contaminates
oysters. Predisposed individuals who consume raw oysters can die within days from sepsis …