Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary

G Kumar, AM Reaume, E Farrell, MR Gaither - PloS one, 2022 - journals.plos.org
Metabarcoding of environmental DNA is increasingly used for biodiversity assessments in
aquatic communities. The efficiency and outcome of these efforts are dependent upon either …

Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks

L Shu, A Ludwig, Z Peng - Ecology and Evolution, 2021 - Wiley Online Library
Environmental DNA (eDNA) techniques refer to utilizing the organisms' DNA extracted from
environment samples to genetically identify target species without capturing actual …

eDNA in a bottleneck: Obstacles to fish metabarcoding studies in megadiverse freshwater systems

JM Jackman, C Benvenuto, I Coscia… - Environmental …, 2021 - Wiley Online Library
The current capacity of environmental DNA (eDNA) to provide accurate insights into the
biodiversity of megadiverse regions (eg, the Neotropics) requires further evaluation to …

Validating environmental DNA metabarcoding for marine fishes in diverse ecosystems using a public aquarium

KC Morey, TJ Bartley, RH Hanner - Environmental DNA, 2020 - Wiley Online Library
Environmental DNA metabarcoding has been widely touted as a powerful tool for monitoring
biodiversity in marine ecosystems. However, this method still requires thorough validation …

[HTML][HTML] Methodology for fish biodiversity monitoring with environmental DNA metabarcoding: The primers, databases and bioinformatic pipelines

F Xiong, L Shu, H Zeng, X Gan, S He, Z Peng - Water Biology and Security, 2022 - Elsevier
Environmental DNA (eDNA) originates from cellular material shed by organisms into aquatic
or terrestrial environments and can be sampled and monitored using metabarcoding …

Harnessing the power of eDNA metabarcoding for the detection of deep-sea fishes

B McClenaghan, N Fahner, D Cote, J Chawarski… - PloS one, 2020 - journals.plos.org
The deep ocean is the largest biome on Earth and faces increasing anthropogenic
pressures from climate change and commercial fisheries. Our ability to sustainably manage …

Comparing environmental metabarcoding and trawling survey of demersal fish communities in the Gulf of St. Lawrence, Canada

SF Afzali, H Bourdages, M Laporte, C Mérot… - Environmental …, 2021 - Wiley Online Library
Biodiversity assessment is an important part of conservation management that ideally can
be accomplished with noninvasive methods without influencing the structure and functioning …

MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Environmental DNA (eDNA) metabarcoding assays to detect invasive invertebrate species in the Great Lakes

KE Klymus, NT Marshall, CA Stepien - PloS one, 2017 - journals.plos.org
Describing and monitoring biodiversity comprise integral parts of ecosystem management.
Recent research coupling metabarcoding and environmental DNA (eDNA) demonstrate that …

Testing the performance of environmental DNA metabarcoding for surveying highly diverse tropical fish communities: A case study from Lake Tanganyika

CJ Doble, H Hipperson, W Salzburger… - Environmental …, 2020 - Wiley Online Library
Abstract Background and Aims Environmental DNA (eDNA) metabarcoding provides a
highly sensitive method of surveying freshwater fish communities, although studies to date …