Mechanistic insights into bacterial AAA+ proteases and protein-remodelling machines
AO Olivares, TA Baker, RT Sauer - Nature Reviews Microbiology, 2016 - nature.com
To maintain protein homeostasis, AAA+ proteolytic machines degrade damaged and
unneeded proteins in bacteria, archaea and eukaryotes. This process involves the ATP …
unneeded proteins in bacteria, archaea and eukaryotes. This process involves the ATP …
AAA+ proteases: ATP-fueled machines of protein destruction
RT Sauer, TA Baker - Annual review of biochemistry, 2011 - annualreviews.org
AAA+ family proteolytic machines (ClpXP, ClpAP, ClpCP, HslUV, Lon, FtsH, PAN/20S, and
the 26S proteasome) perform protein quality control and are used in regulatory circuits in all …
the 26S proteasome) perform protein quality control and are used in regulatory circuits in all …
[HTML][HTML] Stochastic but highly coordinated protein unfolding and translocation by the ClpXP proteolytic machine
JC Cordova, AO Olivares, Y Shin, BM Stinson… - Cell, 2014 - cell.com
ClpXP and other AAA+ proteases recognize, mechanically unfold, and translocate target
proteins into a chamber for proteolysis. It is not known whether these remarkable molecular …
proteins into a chamber for proteolysis. It is not known whether these remarkable molecular …
[HTML][HTML] ClpXP, an ATP-powered unfolding and protein-degradation machine
TA Baker, RT Sauer - Biochimica et Biophysica Acta (BBA)-Molecular Cell …, 2012 - Elsevier
ClpXP is a AAA+ protease that uses the energy of ATP binding and hydrolysis to perform
mechanical work during targeted protein degradation within cells. ClpXP consists of …
mechanical work during targeted protein degradation within cells. ClpXP consists of …
[HTML][HTML] AAA+ chaperones and acyldepsipeptides activate the ClpP protease via conformational control
The Clp protease complex degrades a multitude of substrates, which are engaged by a
AAA+ chaperone such as ClpX and subsequently digested by the dynamic, barrel-shaped …
AAA+ chaperone such as ClpX and subsequently digested by the dynamic, barrel-shaped …
[HTML][HTML] Diverse pore loops of the AAA+ ClpX machine mediate unassisted and adaptor-dependent recognition of ssrA-tagged substrates
ClpX, an archetypal proteolytic AAA+ unfoldase, must engage the ssrA tags of appropriate
substrates prior to ATP-dependent unfolding and translocation of the denatured polypeptide …
substrates prior to ATP-dependent unfolding and translocation of the denatured polypeptide …
Protein unfolding by a AAA+ protease is dependent on ATP-hydrolysis rates and substrate energy landscapes
In the AAA+ ClpXP protease, repetitive cycles of ATP hydrolysis by ClpX unfold ssrA-tagged
substrates, which are unraveled vectorially starting at the C-terminal tag and translocated …
substrates, which are unraveled vectorially starting at the C-terminal tag and translocated …
[HTML][HTML] A processive rotary mechanism couples substrate unfolding and proteolysis in the ClpXP degradation machinery
The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair
of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently …
of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently …
[HTML][HTML] Sculpting the proteome with AAA+ proteases and disassembly machines
Abstract Machines of protein destruction—including energy-dependent proteases and
disassembly chaperones of the AAA+ ATPase family—function in all kingdoms of life to …
disassembly chaperones of the AAA+ ATPase family—function in all kingdoms of life to …
Regulated proteolysis in bacteria
SA Mahmoud, P Chien - Annual review of biochemistry, 2018 - annualreviews.org
Regulated proteolysis is a vital process that affects all living things. Bacteria use energy-
dependent AAA+ proteases to power degradation of misfolded and native regulatory …
dependent AAA+ proteases to power degradation of misfolded and native regulatory …