Scaffold-free tissue engineering is desired in creating consistently sized and shaped cell
aggregates but has been limited to spheroid-like structure and function, thus restricting its …

Micropatterned suspension culture creates consistently sized and shaped cell aggregates
but has not produced organotypic structures from stable cells, thus restricting its use in …

Introduction: The lungs are a complex organ with various cell types. Many factors can
contribute to the damage of the lung epithelial cells, including exposure to air pollutants …

Human respiratory disease research currently lacks in vitro models that recapitulate most of
the physiology and architecture of the lung. Furthermore, the complex composition and …

Human lung organoids (HLOs) are enabling the study of human lung development and
disease by modeling native organ tissue structure, cellular composition, and cellular …

This unit describes a protocol for generation of lung organoids. A lung organoid is a 3D
cell/hydrogel composite that resembles the morphology and cellular composition of the …

Introduction: The lung is an important and complicated organ that has profound impacts on
the entire body when impacted by disease or illness. Due to its complexity, the human lung …

Tissue engineering is a dichotomy of scaffold-based and scaffold-free cultures. Scaffold-
based cultures form highly organotypic structures but with low uniformity and throughput …

Organoid 3D systems are powerful platforms to study development and disease. Recently,
the complexity of lung organoid models derived from adult mouse and human stem cells has …

The application of organoid models to understand lung development, study disease, and
investigate regeneration has grown rapidly over the past decade. Formation of more …