Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications

JL Popot, JL Popot - Membrane Proteins in Aqueous Solutions: From …, 2018 - Springer
Tagged amphipols (APols) are obtained by chemically grafting APols with moieties that can
interact with specific partners. They combine in a single molecule three highly useful …

The use of amphipols as universal molecular adapters to immobilize membrane proteins onto solid supports

D Charvolin, JB Perez, F Rouvière… - Proceedings of the …, 2009 - National Acad Sciences
Because of the importance of their physiological functions, cell membranes represent critical
targets in biological research. Membrane proteins, which make up≈ 1/3 of the proteome …

Synthesis of a polyhistidine-bearing amphipol and its use for immobilizing membrane proteins

F Giusti, P Kessler, RW Hansen, EA Della Pia… - …, 2015 - ACS Publications
Amphipols (APols) are short amphipathic polymers that stabilize membrane proteins (MPs)
in aqueous solutions. In the present study, A8–35, a polyacrylate-based APol, was grafted …

Functionalized amphipols: a versatile toolbox suitable for applications of membrane proteins in synthetic biology

EA Della Pia, RW Hansen, M Zoonens… - The Journal of membrane …, 2014 - Springer
Amphipols are amphipathic polymers that stabilize membrane proteins isolated from their
native membrane. They have been functionalized with various chemical groups in the past …

Labeling and functionalizing amphipols for biological applications

C Le Bon, JL Popot, F Giusti - The Journal of membrane biology, 2014 - Springer
Amphipols (APols) are short amphipathic polymers developed as an alternative to
detergents for handling membrane proteins (MPs) in aqueous solution. MPs are, as a rule …

Trapping and stabilization of integral membrane proteins by hydrophobically grafted glucose-based telomers

P Bazzacco, KS Sharma, G Durand, F Giusti… - …, 2009 - ACS Publications
Amphipols (APols) are short amphipathic polymers designed to adsorb onto the
transmembrane surface of membrane proteins, keeping them water-soluble in the absence …

Synthesis of an oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins

C Le Bon, EA Della Pia, F Giusti, N Lloret… - Nucleic acids …, 2014 - academic.oup.com
Amphipols (APols) are specially designed amphipathic polymers that stabilize membrane
proteins (MPs) in aqueous solutions in the absence of detergent. A8–35, a polyacrylate …

[图书][B] Membrane proteins in aqueous solutions: from detergents to amphipols

JL Popot - 2018 - Springer
This book is the first to be entirely devoted to the challenging art of handling membrane
proteins out of their natural environment, a key process in biological and pharmaceutical …

Folding and stability of integral membrane proteins in amphipols

JH Kleinschmidt, JL Popot - Archives of biochemistry and biophysics, 2014 - Elsevier
Amphipols (APols) are a family of amphipathic polymers designed to keep transmembrane
proteins (TMPs) soluble in aqueous solutions in the absence of detergent. APols have …

Amphipol-mediated screening of molecular orthoses specific for membrane protein targets

Y Ferrandez, M Dezi, M Bosco, A Urvoas… - The Journal of …, 2014 - Springer
Specific, tight-binding protein partners are valuable helpers to facilitate membrane protein
(MP) crystallization, because they can i) stabilize the protein, ii) reduce its conformational …