An Efficient Opal‐Suppressor Tryptophanyl Pair Creates New Routes for Simultaneously Incorporating up to Three Distinct Noncanonical Amino Acids into Proteins in …
Site‐specific incorporation of multiple distinct noncanonical amino acids (ncAAs) into
proteins in mammalian cells is a promising technology, where each ncAA must be assigned …
proteins in mammalian cells is a promising technology, where each ncAA must be assigned …
Mutually orthogonal nonsense-suppression systems and conjugation chemistries for precise protein labeling at up to three distinct sites
Site-specific incorporation of multiple distinct noncanonical amino acids (ncAAs) into a
protein is an emerging technology with tremendous potential. It relies on mutually …
protein is an emerging technology with tremendous potential. It relies on mutually …
[HTML][HTML] Defining the current scope and limitations of dual noncanonical amino acid mutagenesis in mammalian cells
The ability to site-specifically incorporate two distinct noncanonical amino acids (ncAAs) into
the proteome of a mammalian cell with high fidelity and efficiency will have many enabling …
the proteome of a mammalian cell with high fidelity and efficiency will have many enabling …
Genetic encoding of three distinct noncanonical amino acids using reprogrammed initiator and nonsense codons
We recently described an orthogonal initiator tRNA (i tRNATy2) that can initiate protein
synthesis with noncanonical amino acids (ncAAs) in response to the UAG nonsense codon …
synthesis with noncanonical amino acids (ncAAs) in response to the UAG nonsense codon …
Expanding the scope of single-and double-noncanonical amino acid mutagenesis in mammalian cells using orthogonal polyspecific leucyl-tRNA synthetases
Y Zheng, R Mukherjee, MA Chin, P Igo… - Biochemistry, 2018 - ACS Publications
Engineered aminoacyl-tRNA synthetase/tRNA pairs that enable site-specific incorporation of
noncanonical amino acids (ncAAs) into proteins in living cells have emerged as powerful …
noncanonical amino acids (ncAAs) into proteins in living cells have emerged as powerful …
Truncation-free genetic code expansion with tetrazine amino acids for quantitative protein ligations
AJ Eddins, RM Bednar, S Jana, AH Pung… - Bioconjugate …, 2023 - ACS Publications
Quantitative labeling of biomolecules is necessary to advance areas of antibody–drug
conjugation, super-resolution microscopy imaging of molecules in live cells, and …
conjugation, super-resolution microscopy imaging of molecules in live cells, and …
[HTML][HTML] Site-specific incorporation of two ncAAs for two-color bioorthogonal labeling and crosslinking of proteins on live mammalian cells
The pyrrolysyl-tRNA/pyrrolysyl-tRNA synthetase (PylT/RS) pair from the archaeon
Methanosarcina mazei (Mma) is widely used in protein engineering to site-specifically …
Methanosarcina mazei (Mma) is widely used in protein engineering to site-specifically …
[HTML][HTML] Noncanonical amino acids as doubly bio-orthogonal handles for one-pot preparation of protein multiconjugates
Y Wang, J Zhang, B Han, L Tan, W Cai, Y Li… - Nature …, 2023 - nature.com
Genetic encoding of noncanonical amino acid (ncAA) for site-specific protein modification
has been widely applied for many biological and therapeutic applications. To efficiently …
has been widely applied for many biological and therapeutic applications. To efficiently …
Site-specific incorporation of a thioester containing amino acid into proteins
Here, we report the site-specific incorporation of a thioester containing noncanonical amino
acid (ncAA) into recombinantly expressed proteins. Specifically, we genetically encoded a …
acid (ncAA) into recombinantly expressed proteins. Specifically, we genetically encoded a …
[PDF][PDF] Genetic incorporation of multiple unnatural amino acids into proteins in mammalian cells.
H Xiao, A Chatterjee, S Choi, KM Bajjuri… - Angewandte Chemie …, 2013 - xiao.rice.edu
The ability to genetically incorporate unnatural amino acids (UAAs) at specific sites in the
proteome of living cells provides a powerful tool to both investigate and engineer protein …
proteome of living cells provides a powerful tool to both investigate and engineer protein …