Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important
forage crops, is challenging due to the lack of a reference genome and an efficient genome …

Alfalfa (Medicago sativa) is an outcrossing tetraploid legume species widely cultivated in the
world. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR …

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

Abstract Main conclusion The CRISPR/Cas9 technique was successfully used to edit the
genome of the obligatory outcrossing plant species Medicago sativa L.(alfalfa). RNA-guided …

Genome editing using CRISPR–Cas9 works efficiently in plant cells, but delivery of genome-
editing machinery into the vast majority of crop varieties is not possible using established …

As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …

Cereals are the main source of human food on our planet. The ever-increasing food
demand, continuously changing environment, and diseases of cereal crops have made …

Common wheat (Triticum aestivum L.) is one of the three major food crops in the world; thus,
wheat breeding programs are important for world food security. Characterizing the genes …

Efficient multiplex genome editing by CRISPR/Cas9 in common wheat - PMC Back to Top Skip
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Common wheat is a major source of nutrition around the globe, but unlike maize and rice
hybrids, no breakthrough has been made to enhance wheat yield since Green Revolution …