The nexus of engineering and molecular biology has given birth to high-throughput
technologies that allow biologists and medical scientists to produce previously unattainable …

With its well-characterized genome, simple anatomy, and vast array of uses in molecular
biology, the roundworm, Caenorhabditis elegans (C. elegans) is a well-established model …

A thorough understanding of nerve regeneration in Caenorhabditis elegans requires
performing femtosecond laser nanoaxotomy while minimally affecting the worm. We present …

Several sophisticated microfluidic devices have recently been proposed for femtosecond
laser axotomy in the nematode C. elegans for immobilization of the animals for surgery to …

The nematode Caenorhabditis elegans is a widely adopted model organism for studying
various neurobiological processes at the molecular and cellular level in vivo. With a small …

Femtosecond laser nanosurgery has been widely accepted as an axonal injury model,
enabling nerve regeneration studies in the small model organism, Caenorhabditis elegans …

Laser axotomy followed by time-lapse microscopy is a sensitive assay for axon regeneration
phenotypes in C. elegans1. The main difficulty of this assay is the perceived cost ($25-100K) …

High-content time-lapse assays on whole animals require their repeated immobilization for
high-resolution imaging and manipulation. Here, we present a simple, rapid, and minimally …

Our understanding of nerve regeneration can be enhanced by delineating its underlying
molecular activities at single-neuron resolution in model organisms such as Caenorhabditis …

High-resolution in vivo time-lapse assays require repeated immobilization and imaging of
whole animals. Here we report a technology for screening Caenorhabditis elegans at …