Background Nanoinjection—the process of intracellular delivery using vertically configured
nanostructures—is a physical route that efficiently negotiates the plasma membrane, with …

Transfection is a critical step for gene editing and cell‐based therapies. Nanoscale
technologies have shown great promise in providing higher transfection efficiency and lower …

Nanochannel electroporation (NEP) is a new technology for cell transfection, which provides
superior gene delivery and cell viability to conventional bulk electroporation (BEP). In NEP …

The ability to deliver foreign cargos into single living cells is of great interest in cell biology
and therapeutic research. Here, we have reported a single or multiple position based nano …

Intracellular delivery of functional molecules, such as DNA probes and plasmids, is an
important method for investigating cellular mechanisms and changing cell fates in …

Insertion of foreign molecules such as functionalized fluorescent probes, antibodies, or
plasmid DNA to living cells requires overcoming the plasma membrane barrier without …

We introduce a localized single-cell membrane nano-electroporation with controllable
sequential molecular delivery by millisecond to nanosecond electrical pulses. An intense …

Nondestructive introduction of genes, proteins, and small molecules into mammalian cells
with high efficiency is a challenging, yet critical, process. Here we demonstrate a simple …

Intracellular delivery is a critical step in biological discoveries and has been widely utilized
in biomedical research. A variety of molecular tools have been developed for cell-based …

Intracellular delivery of mRNA, DNA, and other large macromolecules into cells plays an
essential role in an array of biological research and clinical therapies. However, current …