Recent advances in the technologies for genomic sequencing and systems for handling and
processing sequencing data have transformed bacterial genomics into a near-routine …

Summary Next-generation sequencing (NGS), also referred to as deep, high-throughput or
massively parallel sequencing, is a powerful new tool that can be used for the complex …

The sum of unique genes in all genomes of a bacterial species is referred to as the pan-
genome and is comprised of variably absent or present accessory genes and universally …

Modern DNA technology facilitates the complete elucidation of bacterial genomes and the
various technologies now in place have been optimized and automated extensively. This …

In the present chapter, we discuss DNA-based typing methods for microbial pathogens that
were frequently used in the past two decades and their essential features, as well as virtues …

The field of cell biology was transformed in 1995 when the complete 1.8 Mb genome of
Haemophilus influenzae was sequenced by The Institute for Genomic Research (TIGR) in …

Since the introduction of the first molecular tests, there has been a continuous effort to
develop new and improved assays for rapid and efficient detection of infectious agents. This …

Terrestrial, aquatic, and aerial animals, either domestic or wild, humans, and plants all face
similar health threats caused by infectious agents. Multifaceted anthropic pressure caused …

New sequencing technologies have made the production of bacterial genome sequences
increasingly easy, and it can be confidently forecasted that vast genomic databases will be …

This chapter focuses on the use of newer sequence‐based methods for discovering novel
microbial pathogens and some of the questions raised concerning interpretation of …