Base editors have been reported to induce off-target mutations in cultured cells, mouse
embryos and rice, but their long-term effects in vivo remain unknown. Here, we develop a …

Cytosine and adenine base editors (CBEs and ABEs) are promising new tools for achieving
the precise genetic changes required for disease treatment and trait improvement. However …

Deaminase base editing has emerged as a tool to install or correct point mutations in the
genomes of living cells in a wide range of organisms. However, the genome-wide off-target …

Two recent articles 1, 2 published in Science separately reported, for the first time, that a
cytosine base editor but not an adenine base editor could induce considerable off-target …

Base editing directly converts a target base pair into a different base pair in the genome of
living cells without introducing double-stranded DNA breaks. While cytosine base editors …

Background Base editors are a class of genome editing tools with the ability to efficiently
induce point mutations in genomic DNA, without inducing double-strand breaks or relying on …

Many human genetic diseases are caused by pathogenic single nucleotide mutations.
Animal models are often used to study these diseases where the pathogenic point mutations …

Base editors (BEs) empower the efficient installation of beneficial or corrective point
mutations in crop and human genomes. However, conventional BEs can induce …

Adenine and cytosine base editors (ABE, CBE) allow for precision genome engineering.
Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced …

Cytosine base editors (CBEs) enable targeted C• G-to-T• A conversions in genomic DNA.
Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide …