[HTML][HTML] Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen–thawed sex-sorted and re-frozen–thawed ram spermatozoa

FK Hollinshead, G Evans, KM Evans, SL Catt… - …, 2004 - rep.bioscientifica.com
The characteristics and functional capacity of ram spermatozoa frozen–thawed prior to and
after flow cytometric sorting was assessed after incubation (37° C; 6 h), in vitro fertilisation …

Birth of offspring of pre-determined sex after artificial insemination of frozen–thawed, sex-sorted and re-frozen–thawed ram spermatozoa

SP De Graaf, G Evans, WMC Maxwell, DG Cran… - Theriogenology, 2007 - Elsevier
The fertility of ram spermatozoa cryopreserved prior to, and following, sex-sorting by flow
cytometry was assessed after insemination of mature Merino ewes at a synchronised estrus …

Production of lambs of predetermined sex after the insemination of ewes with low numbers of frozen–thawed sorted X-or Y-chromosome-bearing spermatozoa

FK Hollinshead, JK O'Brien… - Reproduction, Fertility …, 2002 - CSIRO Publishing
The fertilizing ability of sex-sorted frozen–thawed ram spermatozoa was assessed after
insemination of mature Merino ewes at a synchronized oestrus. Ewes were inseminated into …

In vitro and in vivo assessment of functional capacity of flow cytometrically sorted ram spermatozoa after freezing and thawing

FK Hollinshead, L Gillan, JK O'brien… - Reproduction …, 2003 - CSIRO Publishing
The effect of sex sorting and freeze–thawing on the viability and fertility of ram spermatozoa
was investigated in the present study. Non-sorted (control) frozen–thawed spermatozoa had …

In vitro and in vivo survival of bisected sheep embryos derived from frozen-thawed unsorted, and frozen-thawed sex-sorted and refrozen-thawed ram spermatozoa

KM Morton, AM Rowe, WMC Maxwell, G Evans - Theriogenology, 2006 - Elsevier
Ovine IVP embryos were derived from frozen-thawed unsorted and frozen-thawed sex-
sorted spermatozoa that had been refrozen and thawed. The embryos were bisected and …

Embryo production after in vitro fertilization with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm

SL Underwood, R Bathgate, DC Pereira, A Castro… - Theriogenology, 2010 - Elsevier
The objective of this study was to determine the in vitro fertilizing capacity of bull sperm
derived from fresh or frozen samples and subjected to sex sorting and re-cryopreservation …

Birth of Kids After Artificial Insemination with Sex‐Sorted, Frozen‐Thawed Goat Spermatozoa

R Bathgate, N Mace, K Heasman… - … in Domestic Animals, 2013 - Wiley Online Library
Contents Successful sex‐sorting of goat spermatozoa and subsequent birth of pre‐sexed
kids have yet to be reported. As such, a series of experiments were conducted to develop …

Successful low dose insemination of flow cytometrically sorted ram spermatozoa in sheep

SP De Graaf, G Evans, WMC Maxwell… - … in Domestic Animals, 2007 - Wiley Online Library
Contents The fertility of ram spermatozoa that had undergone flow cytometric sorting
(MoFlo® SX) and cryopreservation was assessed after low‐dose insemination of …

Flow cytometric sorting of frozen–thawed spermatozoa in sheep and non-human primates

JK O'Brien, FK Hollinshead, KM Evans… - Reproduction …, 2004 - CSIRO Publishing
Research was conducted in sheep to determine an effective preparation method for high-
purity sorting of frozen–thawed spermatozoa. The efficacy of sorting frozen–thawed …

Caprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders

L Keskintepe, AA Simplicio, BG Brackett - Theriogenology, 1998 - Elsevier
The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season
was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice …