Precision-cut slices of in vivo tumours permit interrogation in vitro of heterogeneous cells
from solid tumours together with their native microenvironment. They offer a low throughput …

In cancer research and personalized medicine, new tissue culture models are needed to
better predict the response of patients to therapies. With a concern for the small volume of …

The impact of systemic therapy on the tumor microenvironment has been difficult to study in
human solid tumors. Our protocol describes steps for establishing slice cultures to …

Diagnostic and prognostic indicators are key components to achieve the goal of
personalized cancer therapy. Two distinct approaches to this goal include predicting …

Malignant tumor tissues exhibit inter-and intratumoral heterogeneities, aberrant
development, dynamic stromal composition, diverse tissue phenotypes, and cell populations …

Tumors contain heterogeneous and dynamic populations of cells that do not all display the
fast-proliferating properties that traditional chemotherapies target. There is a need therefore …

Abstract Two-dimensional (2D) culture of cancer cells in vitro does not recapitulate the three-
dimensional (3D) architecture, heterogeneity and complexity of human tumors. More …

Current methods to assess the drug response of individual human cancers are often
inaccurate, costly, or slow. Functional approaches that rapidly and directly assess the …

Background The tumor microenvironment (TME) is critical to every aspect of cancer biology.
Organotypic tumor slice cultures (TSCs) preserve the original TME and have demonstrated …

This protocol describes how to build and implement a three-dimensional (3D) cell culture
system, TRACER (tissue roll for analysis of cellular environment and response), that enables …