Artificial transformation methodologies for improving the efficiency of plasmid DNA transformation and simplifying its use
The uptake of exogenous DNA materials through the cell membrane by bacteria, known as
transformation, is essential for the genetic manipulation of bacteria and, thus, plays key roles …
transformation, is essential for the genetic manipulation of bacteria and, thus, plays key roles …
Optimization of chemico-physical transformation methods for various bacterial species using diverse chemical compounds and nanomaterials
Bacterial transformation is a fundamental technology to deliver engineered plasmids into
bacterial cells, which is essential in industrial protein production, chemical production, etc …
bacterial cells, which is essential in industrial protein production, chemical production, etc …
Combined chemical and physical transformation method with RbCl and sepiolite for the transformation of various bacterial species
DNA transformation that delivers plasmid DNAs into bacterial cells is fundamental in genetic
manipulation to engineer and study bacteria. Developed transformation methods to date are …
manipulation to engineer and study bacteria. Developed transformation methods to date are …
Plasmid uptake by bacteria: a comparison of methods and efficiencies
N Yoshida, M Sato - Applied microbiology and biotechnology, 2009 - Springer
The ability to introduce individual molecules of plasmid DNA into cells by transformation has
been of central importance to the recent rapid advancement of plasmid biology and to the …
been of central importance to the recent rapid advancement of plasmid biology and to the …
Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed
TEV Aune, FL Aachmann - Applied microbiology and biotechnology, 2010 - Springer
Methods for transfer of exogenous DNA into cells are essential for genetics and molecular
biology, and the lack of effective methods hampers research on many different species of …
biology, and the lack of effective methods hampers research on many different species of …
Role of membrane potential on artificial transformation of E. coli with plasmid DNA
The standard method of transformation of Escherichea coli with plasmid DNA involves two
important steps: cells are first suspended in 100mM CaCl2 at 0° C (in which DNA is added) …
important steps: cells are first suspended in 100mM CaCl2 at 0° C (in which DNA is added) …
Strategies for the development of bacterial transformation systems
A Mercenier, BM Chassy - Biochimie, 1988 - Elsevier
An effective transformation system is a prerequisite for facile manipulation of bacteria.
Bacteria may be naturally competent for transformation or may be treated with various …
Bacteria may be naturally competent for transformation or may be treated with various …
[PDF][PDF] High efficiency DNA transformation protocol for Escherichia coli using combination of physico-chemical methods
The present study was designed to achieve high DNA transformation efficiency of E. coli by
using the combination of chemical and physical transformation methods. The effect of low …
using the combination of chemical and physical transformation methods. The effect of low …
Natural Escherichia coli strains undergo cell-to-cell plasmid transformation
A Matsumoto, A Sekoguchi, J Imai, K Kondo… - Biochemical and …, 2016 - Elsevier
Horizontal gene transfer is a strong tool that allows bacteria to adapt to various
environments. Although three conventional mechanisms of horizontal gene transfer …
environments. Although three conventional mechanisms of horizontal gene transfer …
How does plasmid DNA penetrate cell membranes in artificial transformation process of Escherichia coli?
Artificial transformation of Escherichia coli with plasmid DNA in presence of CaCl2 is a
widely used technique in recombinant DNA technology. However, exact mechanism of DNA …
widely used technique in recombinant DNA technology. However, exact mechanism of DNA …