[HTML][HTML] Comparison of electron microscopy, ELISA, real time RT-PCR and insulated isothermal RT-PCR for the detection of Rotavirus group A (RVA) in feces of …

MA Soltan, YL Tsai, PYA Lee, CF Tsai… - Journal of virological …, 2016 - Elsevier
There is no gold standard for detection of Rotavirus Group A (RVA), one of the main causes
of diarrhea in neonatal animals. Sensitive and specific real-time RT-PCR (rtRT-PCR) assays …

Sensitive and specific quantitative detection of rotavirus A by one-step real-time reverse transcription-PCR assay without antecedent double-stranded-RNA …

S Mijatovic-Rustempasic, KI Tam, TK Kerin… - Journal of clinical …, 2013 - Am Soc Microbiol
ABSTRACT A real-time quantitative reverse transcription-PCR (qRT-PCR) assay using the
recombinant thermostable Thermus thermophilus (r Tth) enzyme was developed to detect …

Detection of rotavirus species A, B and C in domestic mammalian animals with diarrhoea and genotyping of bovine species A rotavirus strains

PH Otto, S Rosenhain, MC Elschner, H Hotzel… - Veterinary …, 2015 - Elsevier
Rotaviruses (RVs) are a major cause of neonatal diarrhoea in humans and animals
worldwide. In this study, 425 faecal samples were collected between 1999 and 2013 from …

Comparative detection of rotavirus RNA by conventional RT-PCR, TaqMan RT-PCR and real-time nucleic acid sequence-based amplification

QH Mo, HB Wang, H Tan, BM Wu, ZL Feng… - Journal of virological …, 2015 - Elsevier
Rotavirus is one of the major viral pathogens leading to diarrhea. Diagnosis has been
conducted by either traditional cultural, serological methods or molecular biology …

Nested reverse transcriptase‐polymerase chain reaction for the detection of group A rotaviruses

M Elschner, J Prudlo, H Hotzel, P Otto… - Journal of Veterinary …, 2002 - Wiley Online Library
Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all
species of mammals. In the present study, a nested reverse transcriptase‐polymerase chain …

Comparison of two commercial kits and an in-house ELISA for the detection of equine rotavirus in foal feces

S Miño, A Kern, M Barrandeguy, V Parreño - Journal of virological methods, 2015 - Elsevier
Group A rotaviruses (RVA) are important infectious agents associated with diarrhea in the
young of several animal species including foals. Currently, a variety of diagnosis methods …

Evaluation of different genomic regions of Rotavirus A for development of real time PCR

MS Joshi, SG Deore, AM Walimbe, SS Ranshing… - Journal of virological …, 2019 - Elsevier
The nucleotide alignment of all 11 genes of human Rotavirus A (RVA) strains revealed
suitability of NSP2, NSP3 and VP6 genes for the development of real time PCR (qRT-PCR) …

Broadly reactive TaqMan® assay for real-time RT-PCR detection of rotavirus in clinical and environmental samples

N Jothikumar, G Kang, VR Hill - Journal of virological methods, 2009 - Elsevier
Rotaviruses are enteric pathogens responsible for a significant burden of disease,
especially in children, through person-to-person transmission and exposure to contaminated …

Comparative evaluation of commercially available manual and automated nucleic acid extraction methods for rotavirus RNA detection in stools

MD Esona, S McDonald, S Kamili, T Kerin… - Journal of virological …, 2013 - Elsevier
Rotaviruses are a major cause of viral gastroenteritis in children. For accurate and sensitive
detection of rotavirus RNA from stool samples by reverse transcription-polymerase chain …

Rapid detection and high occurrence of porcine rotavirus A, B, and C by RT-qPCR in diagnostic samples

D Marthaler, N Homwong, K Rossow… - Journal of virological …, 2014 - Elsevier
Rotaviruses are important cause of diarrhea in animals, including humans. Currently,
rotavirus species A, B, C, E, and H (RVA-RVC, RVE, and RVH) have been identified in pigs …