Highlights•The lack of predictive modeling limits biosystems design for biofuel production.•
Automation in build and test accelerates strain engineering for biofuel research.•Microplates …

Engineered biological systems such as genetic circuits and microbial cell factories have
promised to solve many challenges in the modern society. However, the artisanal processes …

The long road from emerging biotechnologies to commercial “green” biosynthetic routes for
chemical production relies in part on efficient microbial use of sustainable and renewable …

Synthetic Biology has overcome many of the early challenges facing the field and is entering
a systems era characterized by adoption of Design-Build-Test-Learn (DBTL) approaches …

The increasing demand for bio-based compounds produced from waste or sustainable
sources is driving biofoundries to deliver a new generation of prototyping biomanufacturing …

Sustainable biofuel alternatives to fossil fuel energy are hampered by recalcitrance and
toxicity of biomass substrates to microbial biocatalysts. To address this issue, we present a …

Highlights•Systems-level insight into production strains enables predictable metabolic
engineering.•Multi-omic studies of production and platform strains are increasingly …

Large-scale data acquisition and analysis are often required in the successful
implementation of the design, build, test, and learn (DBTL) cycle in biosystems design …

Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …

The genomic revolution ushered in an era of discovery and characterization of enzymes
from novel organisms that fueled engineering of microbes to produce commodity and high …