Tag-assisted protein purification is a method of choice for both academic researches and
large-scale industrial demands. Application of the purification tags in the protein production …

Highlights•Compares and contrasts new affinity methods to dominant conventional
methods.•Explains how the popular His tag, despite its age, still dominates this …

The purification of recombinant proteins by affinity chromatography is one of the most
efficient strategies due to the high recovery yields and purity achieved. However, this is …

Protein purification remains a central need for biotechnology. In recent years, a class of
aggregating tags has emerged, which offers a quick, cost‐effective and column‐free …

Affinity tags are highly efficient tools for protein purification. They allow the purification of
virtually any protein without any prior knowledge of its biochemical properties. The use of …

The affinity tags are unique proteins/peptides that are attached at the N-or C-terminus of the
recombinant proteins. These tags help in protein purification. Additionally, some affinity tags …

There is currently no generic, simple, low-cost method for affinity chromatographic
purification of proteins in which the purified product is free of appended tags. Existing …

Protein purification typically involves expressing a recombinant gene comprising a target
protein fused to a suitable affinity tag. After purification, it is often desirable to remove the …

Background The potential applications of protein-engineered functional materials are so
wide and exciting that the interest in these eco-friendly advanced materials will further …

Advancements in peptide fusion technologies to maximize the protein production has taken
a big leap to fulfill the demands of post-genomics era targeting elucidation of …