Key message We summarize recent progress of CRISPR/Cas9-mediated gene targeting in
plants, provide recommendations for designing gene-targeting vectors and highlight the …

Highlights•CRISPR/Cas mediated gene editing is becoming routine for plants.•Much more
can be achieved in future by further applications.•Gene editing will become more precise …

The CRISPR/Cas technology has recently become the tool of choice for targeted genome
modification in plants and beyond. Although CRSIPR/Cas offers a rapid and facile way of …

The CRISPR/C as nuclease is becoming a major tool for targeted mutagenesis in
eukaryotes by inducing double‐strand breaks (DSB s) at pre‐selected genomic sites that are …

Mutagenesis continues to play an essential role for understanding plant gene function and,
in some instances, provides an opportunity for plant improvement. The development of gene …

Gene-editing by CRISPR/Cas systems has revolutionized plant biology by serving as a
functional genomics tool. It has tremendously advanced plant breeding and crop …

CRISPR-Cas9 is a powerful tool for generating targeted mutations and genomic deletions.
However, precise gene insertion or sequence replacement remains a major hurdle before …

Recently, CRISPR/Cas9 technology has emerged as a powerful approach for targeted
genome modification in eukaryotic organisms from yeast to human cell lines. Its successful …

Targeted genome engineering (also known as genome editing) has emerged as an
alternative to classical plant breeding and transgenic (GMO) methods to improve crop …

Genome editing is an approach in which a specific target DNA sequence of the genome is
altered by adding, removing, or replacing DNA bases. Artificially engineered hybrid …