Tunable multiphase dynamics of arginine and lysine liquid condensates

RS Fisher, S Elbaum-Garfinkle - Nature communications, 2020 - nature.com
Liquid phase separation into two or more coexisting phases has emerged as a new
paradigm for understanding subcellular organization, prebiotic life, and the origins of …

Expanding the molecular language of protein liquid–liquid phase separation

S Rekhi, CG Garcia, M Barai, A Rizuan, BS Schuster… - Nature Chemistry, 2024 - nature.com
Understanding the relationship between a polypeptide sequence and its phase separation
has important implications for analysing cellular function, treating disease and designing …

Spatially non-uniform condensates emerge from dynamically arrested phase separation

NA Erkamp, T Sneideris, H Ausserwöger… - Nature …, 2023 - nature.com
The formation of biomolecular condensates through phase separation from proteins and
nucleic acids is emerging as a spatial organisational principle used broadly by living cells …

Biophysical characterization of organelle-based RNA/protein liquid phases using microfluidics

N Taylor, S Elbaum-Garfinkle, N Vaidya, H Zhang… - Soft matter, 2016 - pubs.rsc.org
Living cells contain numerous membrane-less RNA/protein (RNP) bodies that assemble by
intracellular liquid–liquid phase separation. The properties of these condensed phase …

Relationship of sequence and phase separation in protein low-complexity regions

EW Martin, T Mittag - Biochemistry, 2018 - ACS Publications
Liquid–liquid phase separation seems to play critical roles in the compartmentalization of
cells through the formation of biomolecular condensates. Many proteins with low-complexity …

Active learning of the thermodynamics-dynamics trade-off in protein condensates

Y An, MA Webb, WM Jacobs - Science Advances, 2024 - science.org
Phase-separated biomolecular condensates exhibit a wide range of dynamic properties,
which depend on the sequences of the constituent proteins and RNAs. However, it is …

RNA is a critical element for the sizing and the composition of phase-separated RNA–protein condensates

M Garcia-Jove Navarro, S Kashida, R Chouaib… - Nature …, 2019 - nature.com
Liquid–liquid phase separation is thought to be a key organizing principle in eukaryotic cells
to generate highly concentrated dynamic assemblies, such as the RNP granules. Numerous …

The growth rate of DNA condensate droplets increases with the size of participating subunits

S Agarwal, D Osmanovic, MA Klocke, E Franco - ACS nano, 2022 - ACS Publications
Liquid–liquid phase separation (LLPS) is a common phenomenon underlying the formation
of dynamic membraneless organelles in biological cells, which are emerging as major …

Biomolecular condensates formed by designer minimalistic peptides

A Baruch Leshem, S Sloan-Dennison… - Nature …, 2023 - nature.com
Inspired by the role of intracellular liquid-liquid phase separation (LLPS) in formation of
membraneless organelles, there is great interest in developing dynamic compartments …

Biomolecular condensates: new opportunities for drug discovery and RNA therapeutics

BA Conti, M Oppikofer - Trends in Pharmacological Sciences, 2022 - cell.com
Biomolecular condensates organize cellular functions in the absence of membranes. These
membraneless organelles can form through liquid–liquid phase separation coalescing RNA …