Transcription and splicing are functionally coupled, resulting in highly efficient splicing of
RNA polymerase II (RNAP II) transcripts. The mechanism involved in this coupling is not …

The pathway of gene expression in higher eukaryotes involves a highly complex network of
physical and functional interactions among the different machines involved in each step of …

It has been widely accepted that the early spliceosome assembly begins with U1 small
nuclear ribonucleoprotein (U1 snRNP) binding to the 5′ splice site (5′ SS), which is …

Biochemical purification of a pre-mRNA splicing activity from HeLa cells that stimulates distal
alternative 3′ splice sites in a concentration-dependent manner resulted in the …

RNA polymerase II (RNAP II) is responsible for transcription of mRNA precursors in
eukaryotic cells. Recent studies, however, have suggested that RNAP II also participates in …

Although both Ul and U2 snRNPs have been implicated in the splicing process, their
respective roles in the earliest stages of intron recognition and spliceosome assembly are …

The splicing of pre‐mRNAs is an essential step of gene expression in eukaryotes. Introns
are removed from split genes through the activities of the spliceosome, a large ribonuclear …

Pre-mRNA splicing complex assembly is mediated by two specific pre-mRNA-snRNP
interactions: Ul snRNP binds to the 5'splice site and U2 snRNP binds to the branch point …

Members of the serine/arginine-rich (SR) protein family have multiple functions in the pre-
mRNA splicing reaction. In addition to being required for the removal of constitutively spliced …

Alternative splicing of precursor messenger RNAs (pre-mRNAs) is an important mechanism
for the regulation of gene expression. The members of the SR protein family of pre-mRNA …