Assisted reproductive technologies in all mammals are critically dependent on the quality of
the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro …

Assisted reproductive technologies in the pig are critical for the production of genetically
modified pigs as models of human disease and to improve production agriculture. Methods …

In vitro embryo production systems are limited by their inability to consistently produce
embryos with the competency to develop to the blastocyst stage, survive cryopreservation …

Nuclear transfer as originally developed for use in amphibians involved microinjecting a
nucleus directly into the cytoplasm of the oocyte. A major mammalian modification has been …

Porcine in vitro production (IVP) systems, including in vitro maturation (IVM) and in vitro
fertilization (IVF) of oocytes and their subsequent in vitro culture (IVC), have been modified …

A system for somatic cell nuclear transfer (SCNT) was developed and led to the successful
production of GFP-transfected piglets. In experiment 1, two groups of SCNT couplets …

Contents Recent advances in new technologies to produce cloned and genetically modified
pigs involve manipulating oocytes and/or embryos in vitro. Although a great deal of progress …

Nuclear transfer of somatic cells and other biotechnologies applied to embryo production in
vitro yield very few embryos that are competent to become live offspring. Multiple factors can …

Genetically modified pigs have become valuable tools for generating advances in animal
agriculture and human medicine. Importantly, in vitro production and manipulation of …

Recent advances in biotechnology have enabled us to produce cloned and genetically
modified cattle and pigs by manipulating in vitro-produced embryos. However, the efficiency …