[HTML][HTML] Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
The CRISPR/Cas9 system is a robust genome editing technology that works in human cells,
animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 …
animals and plants based on the RNA-programmed DNA cleaving activity of the Cas9 …
Precision genome editing in the CRISPR era
J Salsman, G Dellaire - Biochemistry and cell biology, 2017 - cdnsciencepub.com
With the introduction of precision genome editing using CRISPR–Cas9 technology, we have
entered a new era of genetic engineering and gene therapy. With RNA-guided …
entered a new era of genetic engineering and gene therapy. With RNA-guided …
[HTML][HTML] Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
X Liang, J Potter, S Kumar, N Ravinder… - Journal of …, 2017 - Elsevier
While CRISPR-based gene knock out in mammalian cells has proven to be very efficient,
precise insertion of genetic elements via the cellular homology directed repair (HDR) …
precise insertion of genetic elements via the cellular homology directed repair (HDR) …
[HTML][HTML] Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair
N Savic, FCAS Ringnalda, H Lindsay, C Berk… - elife, 2018 - elifesciences.org
The CRISPR-Cas9 targeted nuclease technology allows the insertion of genetic
modifications with single base-pair precision. The preference of mammalian cells to repair …
modifications with single base-pair precision. The preference of mammalian cells to repair …
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells
The insertion of precise genetic modifications by genome editing tools such as CRISPR-
Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared …
Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared …
[HTML][HTML] Understanding the diversity of genetic outcomes from CRISPR-Cas generated homology-directed repair
BM Sansbury, AM Hewes, EB Kmiec - Communications biology, 2019 - nature.com
As CRISPR-Cas systems advance toward clinical application, it is essential to identify all the
outcomes of gene-editing activity in human cells. Reports highlighting the remarkable …
outcomes of gene-editing activity in human cells. Reports highlighting the remarkable …
[HTML][HTML] Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template
The CRISPR-Cas9 system is a powerful genome-editing tool in which a guide RNA targets
Cas9 to a site in the genome, where the Cas9 nuclease then induces a double-stranded …
Cas9 to a site in the genome, where the Cas9 nuclease then induces a double-stranded …
Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements
CRISPR–Cas9 is poised to become the gene editing tool of choice in clinical contexts. Thus
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
far, exploration of Cas9-induced genetic alterations has been limited to the immediate …
[HTML][HTML] Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
Off-target DNA cleavage is a paramount concern when applying CRISPR-Cas9 gene-editing
technology to functional genetics and human therapeutic applications. Here, we show that …
technology to functional genetics and human therapeutic applications. Here, we show that …
[HTML][HTML] Methods for optimizing CRISPR-Cas9 genome editing specificity
Advances in the development of delivery, repair, and specificity strategies for the CRISPR-
Cas9 genome engineering toolbox are helping researchers understand gene function with …
Cas9 genome engineering toolbox are helping researchers understand gene function with …