Gossypium hirsutum is an allotetraploid species, meaning that mutants that are difficult to be
generated by classical approaches due to gene redundancy. The CRISPR/Cas9 genome …

Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple
copies that belong to At and Dt subgenomes. Sequence similarity is also very high between …

As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …

The complex allotetraploid genome is one of major challenges in cotton for repressing gene
expression. Developing site-specific DNA mutation is the long-term dream for cotton …

The base‐editing technique using CRISPR/nC as9 (Cas9 nickase) or dC as9 (deactivated
Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this …

The application of temperature sensitivity CRISPR/LbCpf1 (LbCas12a) mediated genome
editing in allotetraploid cotton (G. hirsutum) and creation of nontransgenic, gossypol‐free …

Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9
system has been widely used for genome editing in various plants because of its simplicity …

Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …

Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for
genome editing. A new effector with a single nuclease domain, a relatively small size, low …

Alfalfa (Medicago sativa) is an outcrossing tetraploid legume species widely cultivated in the
world. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR …