The acceleration of advances in proteomics has enabled integration with imaging at the EM
and light microscopy levels, cryo-EM of protein structures, and artificial intelligence with …

Biological systems have evolved to utilize proteins to accomplish nearly all functional roles
needed to sustain life. A majority of biological functions occur within the crowded …

Combining diverse experimental structural and interactomic methods allows for the
construction of comprehensible molecular encyclopedias of biological systems. Typically …

Over the next few years, structural proteomics will grapple with the problem of visualizing
increasingly elaborate structures, from the atomic details of protein structures up to …

Traditionally, structural biologists approach the complexity of cellular proteomes in a
reductionist manner. Proteomes are fractionated, their molecular components purified and …

Cryo-electron tomography is an emerging imaging technique that has unique potential for
molecular cell biology. At the present resolution of 4–5 nm, large supramolecular structures …

▪ Abstract Proteomics is a rapidly emerging set of key technologies that are being used to
identify proteins and map their interactions in a cellular context. With the sequencing of the …

The term" proteomics" encompasses the large-scale detection and analysis of proteins and
their post-translational modifications. Driven by major improvements in mass spectrometric …

To increase our current understanding of cellular processes, such as cell signaling and
division, knowledge is needed about the spatial and temporal organization of the proteome …

Highlights•Biophysical methods for studying proteins in situ reached unprecedented levels
of maturity.•Direct investigations of cellular protein structures and functions are now …