Protocols for isolating and amplifying fungal DNA from ectomycorrhizae, overcoming the
presence of PCR inhibitors, were established to ensure a fast and reliable truffle mycorrhizal …

This study reports PCR-based techniques for a reliable molecular identification of five
species of white truffles: Tuber magnatum Pico, T. borchii Vittad., T. maculatum Vittad., T …

DNA analyses were developed to type mycorrhizas of two Tuber species of commercial
value (T. melanosporum, T. borchii) and a competitive fungus (Sphaerosporella brunnea) …

A quantitative real-time PCR (qPCR) marker Ta0 with hydrolysis probe (“TaqMan”), targeted
to the internal transcribed spacer region of the ribosomal DNA, has been developed for …

Tuber rufum is a truffle widely distributed throughout Europe, which forms mycorrhizal
associations with numerous species of broadleaf and coniferous trees. The possibility of T …

Five species of white truffle were classified using PCR-based techniques. RAPD (random
amplified polymorphic DNA) fingerprints and specific pairs of primers were used. A RAPD …

Truffles are hypogeous ectomycorrhizal (EM) fungi belonging to the genus Tuber. Although
outplanting of truffle-inoculated host plants has enabled the realization of productive …

Species-specific internal ITS primers that amplify polymerase chain reaction (PCR) products
of different lengths were selected to distinguish the morphologically similar ectomycorrhizal …

Truffles are hypogeous ectomycorrhizal fungi. They belong to the genus Tuber and are
currently considered a hot spot in fungal biology due to their ecological and economic …

The aims of the work were to elucidate the distribution of the ectomycorrhizal fungus Tuber
magnatum Pico during its symbiotic stage, and to identify the root-associated fungi in a …