CRISPR/Cas9 editing genome of extremophile Halomonas spp.
Extremophiles are suitable chassis for developing the next generation industrial
biotechnology (NGIB) due to their resistance to microbial contamination. However …
biotechnology (NGIB) due to their resistance to microbial contamination. However …
Chromosome engineering of the TCA cycle in Halomonas bluephagenesis for production of copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV)
Abstract Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV) is a promising biopolyester
with good mechanical properties and biodegradability. Large-scale production of PHBV is …
with good mechanical properties and biodegradability. Large-scale production of PHBV is …
[HTML][HTML] Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi
W Tao, L Lv, GQ Chen - Microbial Cell Factories, 2017 - Springer
Background Clustered regularly interspaced short palindromic repeats interference
(CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model …
(CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model …
[HTML][HTML] Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation
Halomonas bluephagenesis, a haloalkaliphilic bacterium and native polyhydroxybutyrate
(PHB) producer, is a non-traditional bioproduction chassis for the next generation industrial …
(PHB) producer, is a non-traditional bioproduction chassis for the next generation industrial …
Promoter Engineering for Enhanced P(3HB-co-4HB) Production by Halomonas bluephagenesis
Promoters for the expression of heterologous genes in Halomonas bluephagenesis are
quite limited, and many heterologous promoters function abnormally in this strain. Pporin, a …
quite limited, and many heterologous promoters function abnormally in this strain. Pporin, a …
[HTML][HTML] A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a
X Ao, Y Yao, T Li, TT Yang, X Dong, ZT Zheng… - Frontiers in …, 2018 - frontiersin.org
Various methods for editing specific sites in the Escherichia coli chromosome are available,
and gene-size (∼ 1 kb) integration into a single site or to introduce deletions, short …
and gene-size (∼ 1 kb) integration into a single site or to introduce deletions, short …
MACBETH: multiplex automated Corynebacterium glutamicum base editing method
Y Wang, Y Liu, J Liu, Y Guo, L Fan, X Ni, X Zheng… - Metabolic …, 2018 - Elsevier
Abstract CRISPR/Cas9 or Cpf1-introduced double strand break dramatically decreases
bacterial cell survival rate, which hampers multiplex genome editing in bacteria. In addition …
bacterial cell survival rate, which hampers multiplex genome editing in bacteria. In addition …
Engineering of Halomonas bluephagenesis for low cost production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) from glucose
Abstract Poly (3-hydroxybutyrate-co− 4-hydroxybutyrate)[P (3HB-co− 4HB)] is one of the
most promising biomaterials expected to be used in a wide range of scenarios. However, its …
most promising biomaterials expected to be used in a wide range of scenarios. However, its …
Engineering low-salt growth Halomonas Bluephagenesis for cost-effective bioproduction combined with adaptive evolution
L Zhang, Y Lin, X Yi, W Huang, Q Hu, Z Zhang… - Metabolic …, 2023 - Elsevier
Halophilic Halomonas bluephagenesis has been engineered to produce various added-
value bio-compounds with reduced costs. However, the salt-stress regulatory mechanism …
value bio-compounds with reduced costs. However, the salt-stress regulatory mechanism …
Engineering Halomonas TD01 for the low-cost production of polyhydroxyalkanoates
The halophile Halomonas TD01 and its derivatives have been successfully developed as a
low-cost platform for the unsterile and continuous production of chemicals. Therefore, to …
low-cost platform for the unsterile and continuous production of chemicals. Therefore, to …