Overview of the different methods for RNA preparation in COVID-19 diagnosis process during the pandemic

F Shahi, M Rasti, M Moradi - Analytical Biochemistry, 2023 - Elsevier
The COVID-19 pandemic brought to light the impact of a widespread disease on various
aspects of human relationships, communities, and economies. One notable consequence …

Comparison of different kits for SARS-CoV-2 RNA extraction marketed in Brazil

AKA Eisen, M Demoliner, JS Gularte, AW Hansen… - BioRxiv, 2020 - biorxiv.org
December 2019 marked the begining of the greatest pandemic since Spanish Flu, the
disease named Covid-19 that cause severe pneumonia. Until May 19, 2020 more than 4 …

[HTML][HTML] A rapid One-Pot RNA isolation method for simplified clinical detection of SARS-COV-2 infection in India

S Jain, A Bhowmick, AK Pandey - Frontiers in Cellular and Infection …, 2022 - frontiersin.org
Background With the rapid increase in COVID-19 cases and the discovery of new viral
variants within India over multiple waves, the expensive reagents and time-consuming …

[HTML][HTML] Regenerated silica-based RNA purification columns to address the short supply of RNA purification kits for COVID-19 diagnosis

JK Biswal, R Ranjan, SS Dahiya, S Mallick… - Molecular Biology …, 2021 - Springer
Background RT-qPCR technique is the current world-wide method used for the early
detection of SARS-CoV2 RNA in the suspected clinical samples. Viral RNA extraction is the …

[HTML][HTML] Evaluation of various alternative economical and high Throughput SARS-CoV-2 Testing Methods within Resource-Limited settings

Z Duma, V Ramsuran, AA Chuturgoon… - International Journal of …, 2022 - mdpi.com
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak posed a
challenge for diagnostic laboratories worldwide, with low-middle income countries (LMICs) …

[HTML][HTML] Sensitive extraction-free SARS-CoV-2 RNA virus detection using a chelating resin

B Guan, KM Frank, JO Maldonado, M Beach, E Pelayo… - Iscience, 2021 - cell.com
Current conventional detection of SARS-CoV-2 involves collection of a patient's sample with
a nasopharyngeal swab, storage of the swab during transport in a viral transport medium …

[HTML][HTML] Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection

F Lázaro-Perona, C Rodriguez-Antolín… - PLoS …, 2021 - journals.plos.org
Background Two automatable in-house protocols for high-troughput RNA extraction from
nasopharyngeal swabs for SARS-CoV-2 detection have been evaluated. Methods One …

[HTML][HTML] Strategies that facilitate extraction-free SARS-CoV-2 nucleic acid amplification tests

DJ Delgado-Diaz, D Sakthivel, HHT Nguyen… - Viruses, 2022 - mdpi.com
The COVID-19 pandemic has resulted in an unprecedented global demand for in vitro
diagnostic reagents. Supply shortages and hoarding have impacted testing capacity which …

[HTML][HTML] A faster and less costly alternative for RNA extraction of SARS-CoV-2 using proteinase k treatment followed by thermal shock

AM Ñique, F Coronado-Marquina, JA Mendez Rico… - PLoS …, 2021 - journals.plos.org
One of the biggest challenges during the pandemic has been obtaining and maintaining
critical material to conduct the increasing demand for molecular tests. Sometimes, the lack of …

[HTML][HTML] A Comprehensive Comparison of Rapid RNA Extraction Methods for Detection of SARS-CoV-2 as the Infectious Agent of the Upper Respiratory Tract using …

MM Goodarzi, RT Pirposhteh, H Ravan… - Advanced Biomedical …, 2023 - journals.lww.com
Background: The current COVID-19 pandemic has highlighted the need for faster and more
cost-effective diagnostic methods. The RNA extraction step in current diagnostic methods …