Background CRISPR/Cas9-mediated transcriptional interference (CRISPRi) enables
programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene …

Genome-scale CRISPR interference (CRISPRi) is widely utilized to study cellular processes
in a variety of organisms. Despite the dominance of Saccharomyces cerevisiae as a model …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

For more than 100 years, the fruit fly, Drosophila melanogaster, has served as a powerful
model organism for biological and biomedical research due to its many genetic and …

The multifaceted nature of CRISPR screens has propelled advancements in the field of
functional genomics. Pooled CRISPR screens involve creating programmed genetic …

The yeast S. cerevisiae serves as a model organism for many decades. Numerous
molecular tools have been developed throughout the years to engineer its genome …

Genome editing using the CRISPR-Cas system has vastly advanced the ability to precisely
edit the genomes of various organisms. In the context of mammalian cells, this technology …

The versatility of clustered regularly interspaced short palindromic repeat (CRISPR)-
associated (Cas) genome editing makes it a popular tool for many research and …

Recent advances in CRISPR/Cas9 based genome editing have considerably advanced
genetic engineering of industrial yeast strains. In this study, we report the construction and …

Although only 6 years old, the CRISPR system has blossomed into a tool for rapid, on-
demand genome engineering and gene regulation in Saccharomyces cerevisiae. In this …