This chapter discusses the organization of Tn3‐family transposons and their transposition
(the formation and resolution of the cointegrate intermediate). The bulk of the chapter …

Homologous recombination at the bacterial transposon Tn7 donor site is stimulated 10-fold
when Tn7 is activated to transpose at high frequency in RecD-Escherichia coli, where …

Transposon Tn10 inserts preferentially into particular" hotspots" that have been shown by
sequence analysis to contain the symmetrical consensus sequence 5'-GCTNAGC-3'. This …

In the presence of ATP and Mg2+, the bacterial transposon Tn7 translocates via a cut and
paste mechanism executed by the transposon-encoded proteins TnsA+ TnsB+ TnsC+ TnsD …

We have measured the frequency of Tn9 transposition and cointegrate formation in several
different ways and have examined the stability of the cointegrates. We have also physically …

Tn10 transposition is accomplished without extensive replication of the transposon
sequences. Replicative cointegrate formation is precluded by efficient separation of …

We present evidence that excision of the nonreplicative transposon Tn10 involves three
distinct chemical steps, first-strand nicking, hairpin formation, and hairpin resolution. This …

The ampicillin resistance transposon, Tn3, is one of the most extensively studied and best
understood procaryotic transposable DNA elements. It is the prototype of a large class of …

During Tn10 transposition, the element is excised from the donor site by double-strand
cleavages at the two transposon ends. Double-strand cleavage is a central step in the …

The bacterial transposon Tn7uses a cut and paste mechanism to translocate between non-
homologous insertion sites. In the first step of recombination, double-strand breaks at each …