Proteomics, the characterization of the proteome, is conceptually simple but technically
challenging. Development of such technologies as mass spectrometry, multidimensional …

Due to the complex nature of the proteome, instrumentation and methods development for
sample cleanup, fractionation, preconcentration, chromatographic separation and detection …

In this paper, we present a combined top-down/bottom-up proteomic analysis workflow for
the characterization of proteomic samples. This workflow combines protein fractionation …

Multidimensional chromatography coupled to tandem mass spectrometry is an emerging
technique for the analysis of proteomes and is rapidly being implemented by many …

Shotgun proteomics dominates the field of proteomics. The foundations of the strategy
consist of multiple rounds of peptide separation where chromatography provides the …

Proteomics is the large-scale study of proteins, particularly their expression, structures and
functions. This still-emerging combination of technologies aims to describe and characterize …

Current solution based proteomic analysis methods are generally based on enzymatic
digestion of a protein mixture followed by separation using multidimensional liquid …

Multidimensional liquid-based separation techniques are described for maximizing the
resolution of the enormous number of peptides generated upon tryptic digestion of …

As a complementary approach to two-dimensional polyacrylamide gel electrophoresis (2D-
PAGE), multi-dimensional chromatography separation methods have been widely applied in …

The recent development of numerous technologies for proteome analysis holds the promise
of new and more precise methods for disease diagnosis. In this review, we provide an …