Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors
High-throughput genetic screens are powerful methods to identify genes linked to a given
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …
Morphological and transcriptional responses to CRISPRi knockdown of essential genes in Escherichia coli
ABSTRACT CRISPR interference (CRISPRi) has facilitated the study of essential genes in
diverse organisms using both high-throughput and targeted approaches. Despite the …
diverse organisms using both high-throughput and targeted approaches. Despite the …
[PDF][PDF] Mismatch-CRISPRi reveals the co-varying expression-fitness relationships of essential genes in Escherichia coli and Bacillus subtilis
Essential genes are the hubs of cellular networks, but lack of high-throughput methods for
titrating gene expression has limited our understanding of the fitness landscapes against …
titrating gene expression has limited our understanding of the fitness landscapes against …
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to
decipher gene functions and genetic interactions. Here we use a genome-wide library of …
decipher gene functions and genetic interactions. Here we use a genome-wide library of …
Improved sgRNA design in bacteria via genome-wide activity profiling
CRISPR/Cas9 is a promising tool in prokaryotic genome engineering, but its success is
limited by the widely varying on-target activity of single guide RNAs (sgRNAs). Based on the …
limited by the widely varying on-target activity of single guide RNAs (sgRNAs). Based on the …
On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria
The ability to block gene expression in bacteria with the catalytically inactive mutant of Cas9,
known as dCas9, is quickly becoming a standard methodology to probe gene function …
known as dCas9, is quickly becoming a standard methodology to probe gene function …
Phage-Induced Expression of CRISPR-Associated Proteins Is Revealed by Shotgun Proteomics in Streptococcus thermophilus
The CRISPR/Cas system, comprised of clustered regularly interspaced short palindromic
repeats along with their associated (Cas) proteins, protects bacteria and archaea from viral …
repeats along with their associated (Cas) proteins, protects bacteria and archaea from viral …
Genome engineering of virulent lactococcal phages using CRISPR-Cas9
Phages are biological entities found in every ecosystem. Although much has been learned
about them in past decades, significant knowledge gaps remain. Manipulating virulent …
about them in past decades, significant knowledge gaps remain. Manipulating virulent …
Bacterial CRISPR screens for gene function
H Todor, MR Silvis, H Osadnik, CA Gross - Current opinion in microbiology, 2021 - Elsevier
Highlights•Bacterial CRISPR approaches are used for gene editing, repression, and
activation.•CRISPRi, which blocks transcription of targeted genes, is the main bacterial …
activation.•CRISPRi, which blocks transcription of targeted genes, is the main bacterial …
Orthologous CRISPR–Cas9 enzymes for combinatorial genetic screens
Combinatorial genetic screening using CRISPR–Cas9 is a useful approach to uncover
redundant genes and to explore complex gene networks. However, current methods suffer …
redundant genes and to explore complex gene networks. However, current methods suffer …