The bioluminescent luciferin-luciferase reaction is based on the oxidation of D-luciferin by
oxygen in the presence of ATP and magnesium ions, catalyzed by firefly luciferase. The …

Theoretical aspects of the adenosine triphosphate bioluminescence assay based on the use
of the firefly luciferin–luciferase system are considered, as well as its application for …

The effects of inorganic salts (sodium and potassium acetate, magnesium and sodium
sulfate, sodium chloride, potassium nitrate, sodium phosphate, sodium and magnesium …

Application of chemiluminescence (CL) for study of free-radical reactions in human and
animal cells and tissues is considered in this review. Historically, the study of intrinsic …

A possibility of using immobilized Luciola mingrelica luciferase for quantitative test of ATP
and ATP synthetizing and degrading enzymes activities is demonstrated. A kinetic scheme is …

The possible applications of the immobilized bioluminescent systems of bacteria and fireflies
in microassay are shown. The immobilization resulted in 10-100-fold stabilization of …

Recent data from gene engineering, kinetic and fluorescent studies concerning the structure
and functions of firefly luciferase are reviewed. Some new trends in the development of …

The review of modern luminescent methods of research, their prospects and potentialities of
practical application in biological researches is presented. Special attention is given to the …

The absorption and fluorescence spectra of dimethyloxyluciferin (DMOL) and
monomethyloxyluciferin (MMOL) were studied at pH 3.0–12.0 In the range of pH 3.0–8.0, the …

The role of SH-groups of the enzyme in the catalytic and inactivation processes of soluble
and immobilized on Sepharose 4B luciferase from the fireflies Luciola mingrelica was …