Much of contemporary synthetic biology research relies on the use of bacterial chassis for
plugging-in and plugging-out genetic circuits and new-to-nature functionalities. However …

Biotechnological production in bacteria enables access to numerous valuable chemical
compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well …

Developing cost-efficient biotechnological processes is a major challenge in replacing fossil-
based industrial production processes. The remarkable progress in genetic engineering …

Metabolic diversity in microorganisms can provide the basis for creating novel biochemical
products. However, most metabolic engineering projects utilize a handful of established …

The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

Highlights•SynBio needs chassis with superior genetic, biochemical, and physiological
traits.•Environmental bacteria are endowed with many advantageous metabolic properties.• …

Microbial chemical production is a rapidly growing industry, with much of the growth fueled
by advances in synthetic biology. New approaches have enabled rapid strain engineering …

Highlights•Pseudomonas species are well suited as a platform for industrial and
environmental biotechnology.•A range of Synthetic Biology methods for genetic refactoring …

Non‐model microorganisms have been increasingly explored as microbial cell factories for
production of chemicals, fuels, and materials owing to their unique physiology and metabolic …