RNA has important and diverse roles in biology, but molecular tools to manipulate and
measure it are limited. For example, RNA interference,, can efficiently knockdown RNAs, but …

CRISPR–Cas interference is mediated by Cas effector nucleases that are either components
of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single …

The CRISPR-associated protein Cas9 is an RNA-guided DNA endonuclease that uses RNA–
DNA complementarity to identify target sites for sequence-specific double-stranded DNA …

The RNA-guided CRISPR-associated protein Cas9 is used for genome editing,
transcriptional modulation, and live-cell imaging. Cas9-guide RNA complexes recognize …

Robust and precise transcript targeting in mammalian cells remains a difficult challenge
using existing approaches due to inefficiency, imprecision and subcellular …

Noncoding RNAs play diverse roles throughout biology and exhibit broad functional
capacity. To investigate and harness these capabilities, we developed clustered regularly …

Bacterial abortive-infection systems limit the spread of foreign invaders by shutting down or
killing infected cells before the invaders can replicate 1, 2. Several RNA-targeting CRISPR …

Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating,
perturbing, and engineering cellular systems. Here, we develop a method for controlling …

CRISPR–Cas systems provide microbes with adaptive immunity by employing short DNA
sequences, termed spacers, that guide Cas proteins to cleave foreign DNA,. Class 2 …

DNA-targeting CRISPR-Cas systems, such as those employing the RNA-guided Cas9 or
Cas12 endonucleases, have revolutionized our ability to predictably edit genomes and …