The regulatory logic of m‐xylene biodegradation by Pseudomonas putida mt‐2 exposed by dynamic modelling of the principal node Ps/Pr of the TOL plasmid

M Koutinas, MC Lam, A Kiparissides… - Environmental …, 2010 - Wiley Online Library
The structure of the extant transcriptional control network of the TOL plasmid pWW0 born by
Pseudomonas putida mt‐2 for biodegradation of m‐xylene is far more complex than one …

The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene

R Silva-Rocha, H de Jong, J Tamames… - BMC Systems Biology, 2011 - Springer
Background The genetic network of the TOL plasmid pWW0 of the soil bacterium
Pseudomonas putida mt-2 for catabolism of m-xylene is an archetypal model for …

Inferring the genetic network of m‐xylene metabolism through expression profiling of the xyl genes of Pseudomonas putida mt‐2

F Velázquez, V Parro, V de Lorenzo - Molecular microbiology, 2005 - Wiley Online Library
A subgenomic array of structural and regulatory genes of the TOL plasmid pWW0 of
Pseudomonas putida mt‐2 has been constructed to sort out the interplay between m‐xylene …

Stochasticity of TOL plasmid catabolic promoters sets a bimodal expression regime in Pseudomonas putida mt‐2 exposed to m‐xylene

R Silva‐Rocha, V de Lorenzo - Molecular microbiology, 2012 - Wiley Online Library
The expression dynamics of the catabolic promoters of the TOL plasmid pWW0 has been
examined in single cells of Pseudomonas putida mt‐2 exposed to m‐xylene. To this end, we …

High‐resolution analysis of the m‐xylene/toluene biodegradation subtranscriptome of Pseudomonas putida mt‐2

J Kim, D Pérez‐Pantoja, R Silva‐Rocha… - Environmental …, 2016 - Wiley Online Library
P seudomonas putida mt‐2 metabolizes m‐xylene and other aromatic compounds through
the enzymes encoded by the xyl operons of the TOL plasmid p WW0 along with other …

Improving the prediction of Pseudomonas putida mt-2 growth kinetics with the use of a gene expression regulation model of the TOL plasmid

M Koutinas, A Kiparissides, MC Lam… - Biochemical …, 2011 - Elsevier
The molecular and genetic events responsible for the growth kinetics of a microorganism
can be extensively influenced by the presence of mixtures of substrates leading to unusual …

Transcriptional induction kinetics from the promoters of the catabolic pathways of TOL plasmid pWW0 of Pseudomonas putida for metabolism of aromatics

S Marqués, A Holtel, KN Timmis… - Journal of bacteriology, 1994 - Am Soc Microbiol
We determined, under several growth conditions, the kinetics of mRNA synthesis from the
four Pseudomonas putida pWW0 plasmid promoters involved in degradation of xylenes and …

Transcriptional kinetics of the cross-talk between the ortho-cleavage and TOL pathways of toluene biodegradation in Pseudomonas putida mt-2

A Tsipa, M Koutinas, EN Pistikopoulos… - Journal of …, 2016 - Elsevier
The TOL plasmid promoters are activated by toluene leading to gene expression
responsible for the degradation of the environmental signal. Benzoate is formed as an …

The m‐xylene biodegradation capacity of Pseudomonas putida mt‐2 is submitted to adaptation to abiotic stresses: evidence from expression profiling of xyl genes

F Velázquez, V De Lorenzo… - Environmental …, 2006 - Wiley Online Library
The effect of archetypal environmental stresses on expression of the catabolic xyl genes of
the TOL plasmid pWW0 of the m‐xylene degrading strain Pseudomonas putida mt‐2 has …

Integration of Signals through Crc and PtsN in Catabolite Repression of Pseudomonas putida TOL Plasmid pWW0

I Aranda-Olmedo, JL Ramos… - Applied and …, 2005 - Am Soc Microbiol
Toluene degradation in Pseudomonas putida KT2440 pWW0 plasmid is subjected to
catabolite repression. Pu and PS1 promoters of the pWW0 TOL plasmid are down-regulated …