Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution
Background Species-level genetic characterization of complex bacterial communities has
important clinical applications in both diagnosis and treatment. Amplicon sequencing of the …
important clinical applications in both diagnosis and treatment. Amplicon sequencing of the …
Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing
Demands for faster and more accurate methods to analyze microbial communities from
natural and clinical samples have been increasing in the medical and healthcare industry …
natural and clinical samples have been increasing in the medical and healthcare industry …
Nanopore is preferable over Illumina for 16S amplicon sequencing of the gut microbiota when species-level taxonomic classification, accurate estimation of richness …
M Szoboszlay, L Schramm, D Pinzauti, J Scerri… - Microorganisms, 2023 - mdpi.com
Nanopore sequencing is a promising technology used for 16S rRNA gene amplicon
sequencing as it can provide full-length 16S reads and has a low up-front cost that allows …
sequencing as it can provide full-length 16S reads and has a low up-front cost that allows …
[HTML][HTML] Microbiota profiling with long amplicons using Nanopore sequencing: full-length 16S rRNA gene and the 16S-ITS-23S of the rrn operon
A Cuscó, C Catozzi, J Viñes, A Sanchez… - …, 2018 - ncbi.nlm.nih.gov
Background: Profiling the microbiome of low-biomass samples is challenging for
metagenomics since these samples are prone to contain DNA from other sources (eg host or …
metagenomics since these samples are prone to contain DNA from other sources (eg host or …
Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
S Kai, Y Matsuo, S Nakagawa, K Kryukov… - FEBS open …, 2019 - Wiley Online Library
Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic
treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene …
treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene …
Evaluation of PCR conditions for characterizing bacterial communities with full-length 16S rRNA genes using a portable nanopore sequencer
S Fujiyoshi, A Muto-Fujita, F Maruyama - Scientific Reports, 2020 - nature.com
Abstract MinION (Oxford Nanopore Technologies), a portable nanopore sequencer, was
introduced in 2014 as a new DNA sequencing technology. MinION is now widely used …
introduced in 2014 as a new DNA sequencing technology. MinION is now widely used …
Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions
High-throughput molecular technologies can profile microbial communities at high
resolution even in complex environments like the intestinal microbiota. Recent …
resolution even in complex environments like the intestinal microbiota. Recent …
Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer
A Benítez-Páez, KJ Portune, Y Sanz - Gigascience, 2016 - academic.oup.com
Background The miniaturised and portable DNA sequencer MinION™ has been released to
the scientific community within the framework of an early access programme to evaluate its …
the scientific community within the framework of an early access programme to evaluate its …
Emu: species-level microbial community profiling of full-length 16S rRNA Oxford Nanopore sequencing data
Abstract 16S ribosomal RNA-based analysis is the established standard for elucidating the
composition of microbial communities. While short-read 16S rRNA analyses are largely …
composition of microbial communities. While short-read 16S rRNA analyses are largely …
Multi-locus and long amplicon sequencing approach to study microbial diversity at species level using the MinION™ portable nanopore sequencer
A Benítez-Páez, Y Sanz - Gigascience, 2017 - academic.oup.com
The miniaturized and portable DNA sequencer MinION™ has demonstrated great potential
in different analyses such as genome-wide sequencing, pathogen outbreak detection and …
in different analyses such as genome-wide sequencing, pathogen outbreak detection and …
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