qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection
and quantification of RNA targets and is being utilized increasingly in novel clinical …

Molecular methods, essentially based upon PCR, have become an indispensable tool in the
diagnosis of infectious diseases. Real-time quantitative PCR (qrtPCR), as a novel …

This chapter analyzes the quantification strategies in real-time RT-PCR and all
corresponding markers of a successful real-time RT-PCR. The following aspects are …

In this paper we determine the limits and accuracy of quantitative reverse transcription (RT)-
PCR using a modification of the original protocol. The quantification of mRNA with this …

The development of the polymerase chain reaction (PCR), for which Kary Mullis received the
1992 Novel Prize in Chemistry, revolutionized molecular biology. At around the time that …

The introduction of real-time PCR technology has significantly improved and simplified the
quantification of nucleic acids, and this technology has become an invaluable tool for many …

Real-time fluorescence-based quantitative PCR has become established as the benchmark
technology for the quantification of nucleic acids, offering an immense choice of protocols …

Molecular diagnostics is one of the major growth areas of modern medicine, with real-time
PCR established as a qualitative and quantitative technology that is rapid, accurate and …

Quantitative real-time polymerase chain reaction (QRT-PCR) has become an extensively
applied technique. It enables quantitative analyses of gene expression applicable to basic …

Abstract Background: Quantitative PCR (qPCR) is a valuable technique for accurately and
reliably profiling and quantifying gene expression. Typically, samples obtained from the …