[HTML][HTML] Factors involved in heterologous expression of proteins in E. coli host

M Pouresmaeil, S Azizi-Dargahlou - Archives of Microbiology, 2023 - Springer
The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …

Strategies for the Production of Recombinant Protein in Escherichia coli

GJ Gopal, A Kumar - The protein journal, 2013 - Springer
In the recent past years, a large number of proteins have been expressed in Escherichia coli
with high productivity due to rapid development of genetic engineering technologies. There …

[HTML][HTML] Recombinant protein expression in Escherichia coli: advances and challenges

GL Rosano, EA Ceccarelli - Frontiers in microbiology, 2014 - frontiersin.org
Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

Practical protocols for production of very high yields of recombinant proteins using Escherichia coli

A Sivashanmugam, V Murray, C Cui, Y Zhang… - Protein …, 2009 - Wiley Online Library
The gram‐negative bacterium Escherichia coli offers a mean for rapid, high yield, and
economical production of recombinant proteins. However, high‐level production of …

Strategies for optimizing heterologous protein expression in Escherichia coli

G Hannig, SC Makrides - Trends in biotechnology, 1998 - cell.com
The many advantages of Escherichia coli have ensured that it remains a valuable host for
the efficient, cost-effective and high-level production of heterologous proteins. Here, we …

New tools for recombinant protein production in Escherichia coli: A 5‐year update

GL Rosano, ES Morales, EA Ceccarelli - Protein science, 2019 - Wiley Online Library
The production of proteins in sufficient amounts is key for their study or use as biotherapeutic
agents. Escherichia coli is the host of choice for recombinant protein production given its fast …

Strategies for optimization of heterologous protein expression in E. coli: Roadblocks and reinforcements

J Kaur, A Kumar, J Kaur - International journal of biological …, 2018 - Elsevier
E. coli is most preferred system used for the production of recombinant proteins in bacteria
and the availability of improved genetic tools/methods are making it more valuable than …

Increasing recombinant protein production in Escherichia coli through metabolic and genetic engineering

H Waegeman, W Soetaert - Journal of Industrial Microbiology …, 2011 - academic.oup.com
Different hosts have been used for recombinant protein production, ranging from simple
bacteria, such as Escherichia coli and Bacillus subtilis, to more advanced eukaryotes as …

Extracellular recombinant protein production from Escherichia coli

Y Ni, R Chen - Biotechnology letters, 2009 - Springer
Escherichia coli is the most commonly used host for recombinant protein production and
metabolic engineering. Extracellular production of enzymes and proteins is advantageous …

Advanced genetic strategies for recombinant protein expression in Escherichia coli

HP Sørensen, KK Mortensen - Journal of biotechnology, 2005 - Elsevier
Preparations enriched by a specific protein are rarely easily obtained from natural host cells.
Hence, recombinant protein production is frequently the sole applicable procedure. The …