Single-chain variable fragment (scFv) has great prospect in medical therapies and
diagnostic applications due to its binding affinity and low immunogenicity. However, the …

Single-chain variable fragment (scFv) antibodies are widely used as diagnostic and
therapeutic agents or biosensors for a majority of human disease. However, the limitations of …

Background The production of therapeutically active single chain variable fragment (scFv)
antibody is still challenging in E. coli due to the aggregation propensity of recombinant …

Single-chain variable fragment (scFv) is a class of engineered antibodies generated by the
fusion of the heavy (VH) and light chains (VL) of immunoglobulins through a short …

Green fluorescent protein (GFP) is widely used as an excellent expression tag for fusion
proteins, which can improve their expression while preserving their function and native-like …

Fibroblast growth factor receptor 3 (FGFR3) is a noted proto-oncogene involved in the
pathogenesis of many tumors, so more and more studies focus on the potential use of …

Type 1 insulin-like growth factor receptor (IGF-1R) is a promising therapeutic target for
cancer treatment. A single-chain variable fragment (scFv) against human IGF-1R forms …

Single-chain Fv antibodies (scFv), a group of reconstructed molecules with several disulfide
bonds, are prone to aggregate as inclusion bodies, the insoluble species of natural proteins …

LIU, Zixuan et al. Expression and purification of soluble single-chain Fv against human
fibroblast growth factor receptor 3 fused with Sumo tag in Escherichia coli. Electron. J …

Recombinant single-chain variable fragment (scFv) antibodies have wide applications in the
areas of biotechnology and medicine. However, there is currently no universal expression …