Mycoplasma species share a set of features, such as lack of a cell wall, streamlined
genomes, simplified metabolism, and the use of a deviant genetic code, that make them …

The development of advanced genetic tools is boosting microbial engineering which can
potentially tackle wide-ranging challenges currently faced by our society. Here we present …

One remarkable achievement in synthetic biology was the reconstruction of mycoplasma
genomes and their cloning in yeast where they can be modified using available genetic …

Mycoplasmas are successful pathogens that cause debilitating diseases in humans and
various animal hosts. Despite the exceptionally streamlined genomes, mycoplasmas have …

Genome engineering of microorganisms has become a standard in microbial
biotechnologies. Several efficient tools are available for the genetic manipulation of model …

Mycoplasmas are important model organisms for Systems and Synthetic Biology, and are
pathogenic to a wide variety of species. Despite their relevance, many of the tools …

ABSTRACT CRISPR-Cas systems provide bacteria and archaea with adaptive immunity
against viruses and plasmids by the detection and cleavage of invading foreign DNA …

Mycoplasmas are minimal bacteria that infect humans, wildlife, and most economically
relevant livestock species. Mycoplasma infections cause a large range of chronic …

Mycobacterium smegmatis is an important model strain of Mycobacterium for scientific study
because it is non‐pathogenic and grows rapidly. However, research is limited by the low …

Efficient and convenient genetic manipulation of mycobacteria, important microorganisms in
human healthcare and the pharmaceutical industry, is limited. In this study, using a model …